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Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses
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Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: Simultaneous measurements of phototactic and photophobic responses
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| 作成者 |
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| アクセス権 |
metadata only access |
| 権利情報 |
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© 1991 Published by Elsevier Inc.
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| 内容注記 |
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Abstract
A real-time automated method was developed for simultaneous measurements of phototactic orientation (phototaxis) and step-up photophobic response of flagellated microorganisms. Addition of all-trans retinal restored both photoresponses in a carotenoid-deficient mutant strain of Chlamydomonas reinhardtii in a dose-dependent manner. The phototactic orientation was biphasic with respect to both the light intensity and the concentration of retinal. All-trans retinal was more effective than 11-cis retinal to regenerate both photobehavioral responses. Analogs having locked 11-cis configurations and a phenyl ring in the side chain also induced photoresponses, although at concentrations more than two orders of magnitude higher than all-trans retinal. According to the present assay method, the responses were hardly detectable in cells incubated with retinal analogs in which the 13-ene was locked in either its trans or cis configuration. The results strongly suggest that the isomerization of the 13-14 double bond is important for photobehavioral signal transduction and that a single retinal-dependent photoreceptor controls both phototactic and photophobic responses.
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| 出版者 |
Elsevier
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 資源識別子 |
URI
https://ir.soken.ac.jp/records/3862
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| 関連 |
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PMID
1872847
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DOI
https://doi.org/10.1016/0006-291X(91)91031-7
10.1016/0006-291X(91)91031-7
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| 収録誌情報 |
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Biochemical and Biophysical Research Communications
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Biochemical and Biophysical Research Communications
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巻178
号3
開始ページ1273
終了ページ1279
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| コンテンツ更新日時 |
2023-08-18 |
