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タイトル
  • Characterization of arylalkylamine N-acetyltransferase (AANAT) activities and action spectrum for suppression in the band-legged cricket, Dianemobius nigrofasciatus (Orthoptera: Gryllidae)
  • en Characterization of arylalkylamine N-acetyltransferase (AANAT) activities and action spectrum for suppression in the band-legged cricket, Dianemobius nigrofasciatus (Orthoptera: Gryllidae)
作成者
    • 渡辺, 正勝
    • e-Rad 40124226
    • IZAWA, Norimitsu
    • SUZUKI, Takeshi
    • WATANABE, Masakatsu
    • TAKEDA, Makio
アクセス権 metadata only access
権利情報
  • © 2009 Published by Elsevier Inc.
内容注記
  • Abstract Arylalkylamine N-acetyltransferase (AANAT), constituting a large family of enzymes, catalyzes the transacetylation from acetyl-CoA to monoamine substrates, although homology among species is not very high. AANAT in vertebrates is photosensitive and mediates circadian regulation. Here, we analyzed AANAT of the cricket, Dianemobius nigrofasciatus. The central nervous system contained AANAT activity. The optimum pHs were 6.0 (a minor peak) and 10.5 (a major peak) with crude enzyme solution. We analyzed the kinetics at pH 10.5 using the sample containing collective AANAT activities, which we term AANAT. Lineweaver-Burk plot and secondary plot yielded a Km for tryptamine as substrate of 0.42 μM, and a Vmax of 9.39 nmol/mg protein/min. The apparent Km for acetyl-CoA was 59.9 μM and the Vmax was 8.14 nmol/mg protein/min. AANAT of D. nigrofasciatus was light-sensitive. The activity was higher at night-time than at day-time as in vertebrates. To investigate most effective wavelengths on AANAT activity, a series of monochromatic lights was applied (350, 400, 450, 500, 550, 600 and 650 nm). AANAT showed the highest sensitivity to around 450 nm and 550 nm. 450 nm light was more effective than 550 nm light. Therefore, the most effective light affecting AANAT activity is blue light, which corresponds to the absorption spectrum of blue wave (BW)-opsin.
出版者 Elsevier
日付
    Issued2009-04
言語
  • eng
資源タイプ journal article
資源識別子 URI https://ir.soken.ac.jp/records/3865
関連
  • PMID 19168144
  • DOI https://doi.org/10.1016/j.cbpb.2008.12.016 10.1016/j.cbpb.2008.12.016
収録誌情報
    • ISSN 10964959
      • Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology en Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
      • 152 4 開始ページ346 終了ページ351
コンテンツ更新日時 2023-08-18