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タイトル	'Reverse chemical evolution': A new method to search for thermally stable biopolymers en 'Reverse chemical evolution': A new method to search for thermally stable biopolymers
作成者	湯川, 哲之 e-Rad 20110091 MITSUZAWA, Shigenobu YUKAWA, Tetsuyuki
アクセス権	metadata only access
権利情報	SpringerLink(The original publication is available at www.springerlink.com)
内容注記	Abstract The primitive sea on Earth may have had high-temperature and high-pressure conditions similar to those in present-day hydrothermal environments. If life originated in the hot sea, thermal stability of the constituent molecules would have been necessary. Thus far, however, it has been reported that biopolymers hydrolyze too rapidly to support life at temperatures of more than 200 °C. We herein propose a novel approach, called reverse chemical evolution, to search for biopolymers notably more stable against thermal decomposition than previously reported. The essence of the approach is that hydrolysis of a protein or functional RNA (m-, t-, r-RNA) at high temperature and high pressure simulating the ancient sea environment may yield thermally stable peptides or RNAs at higher concentrations than other peptides or RNAs. An experimental test hydrolyzing bovine ribonuclease A in aqueous solution at 205 °C and 25 MPa yielded three prominently stable molecules weighing 859, 1030 and 695 Da. They are thermally some tens or hundreds times more stable than a polyglycine of comparable mass. Sequence analyses of the 859- and 1030-Da molecules revealed that they are a heptapeptide and its homologue, respectively, elongated by two amino acids at the N-terminal region, originally embedded as residues 112–120 in the protein. They consist mainly of hydrophobic amino acids.
出版者	SpringerLink
日付	Issued2003-04
言語	eng
資源タイプ	journal article
資源識別子	URI https://ir.soken.ac.jp/records/3949
関連	DOI https://doi.org/10.1023/A:1024621729149 10.1023/A:1024621729149
収録誌情報	ISSN 0169-6149 Origins of Life and Evolution of Biospheres en Origins of Life and Evolution of Biospheres 巻33 号2 開始ページ163 終了ページ171
コンテンツ更新日時	2023-08-18