一覧に戻る

タイトル
  • Expression of neprilysin in periodontitis-affected gingival tissues
  • en Expression of neprilysin in periodontitis-affected gingival tissues
その他のタイトル
  • 歯周炎罹患歯肉組織におけるネプリライシンの発現
作成者
    • Nezu, Arata
権利情報
  • Elsevier
主題
  • Other Gene expression
  • Other Immunolocalisation
  • Other Periodontitis
  • Other Alzheimer’s disease
  • Other Amyloid beta precursor protein
  • Other Neprilysin
内容注記
  • Abstract Objective: Although the pathogeneses of Alzheimer’s disease (AD) and periodontal diseases have overlapping features, including ageing and chronic inflammation, the association between AD and periodontitis remains unclear. To explore the pathogenesis of periodontitis, a comprehensive gene expression/transcriptome analysis in periodontitis-affected gingival tissues found that the AD pathway was significantly up-regulated in periodontitis-affected gingival tissues. AD-related genes, amyloid beta precursor protein (APP), interleukin-1 beta and compliment 1QA, were significantly elevated in periodontitis. In the present study, balance between mRNA expression of APP and a potent amyloid degradation enzyme, neprilysin (NEP), as well as protein localisation of APP and NEP were analysed. Design: Eighteen periodontitis-affected and 18 clinically healthy control gingival tissues were taken from patients with severe chronic periodontitis or undergoing tooth extraction. Total RNA was purified and used for quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR). The localisation of APP and NEP was analysed by immunohistochemistry (IHC). Results: Both APP and NEP genes were up-regulated in periodontitis-affected gingival tissues. APP- expressing macrophages and NEP-expressing neutrophils and fibroblasts, reflecting inflammatory stages, were detected in inflamed gingival tissues by IHC. Conclusion: The up-regulation of APP and NEP mRNA levels in periodontitis-affected gingival tissues compared with healthy controls was confirmed by qRT-PCR analyses. Since NEP is one of the primary enzymes that degrades amyloid beta, increased NEP mRNA levels in periodontitis may act as an inhibitor of amyloid beta accumulation in gingival tissues, balancing increased APP mRNA expression. However, NEP has several effects including degradation of vasoactive substances; therefore, further sresearch is needed.
  • Other 学位の種類: 博士（歯学）. 報告番号: 甲第4439号. 学位記番号: 新大院博（歯）甲第402号. 学位授与年月日: 平成30年3月23日
  • Other Archives of Oral Biology. 2017, 79, 35-41.
  • Other 新大院博（歯）甲第402号
出版者 新潟大学
日付
    Issued2018-03-23
言語
  • eng
資源タイプ thesis
資源識別子 HDL http://hdl.handle.net/10191/50264 , URI https://niigata-u.repo.nii.ac.jp/records/6056
関連
  • DOI https://doi.org/10.1016/j.archoralbio.2017.03.003
収録誌情報
  • 開始ページ35 終了ページ41
学位情報
  • 学位授与番号 13101甲第4439号
  • 学位授与機関
    • 機関名称 新潟大学
  • 学位授与年月日 2018-03-23
  • 学位名 博士（歯学）
ファイル
    • 本文
    • 2.4 MB (application/pdf)
      • Available2
    • 要旨
    • 121.2 kB (application/pdf)
      • Available2
コンテンツ更新日時 2023-06-26