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タイトル	Altered Induction of Reactive Oxygen Species by X-rays in Hematopoietic Cells of C57BL/6-Tg (CAG-EGFP) Mice
作成者	Liu, Cuihua Hirokazu, Hirakawa Takanori, Katsube Fang, YaQun Kaoru, Tanaka Mitsuru, Nenoi Akira, Fujimori Bing, Wang en Liu, Cuihua en Hirokazu, Hirakawa en Takanori, Katsube en Fang, YaQun en Kaoru, Tanaka en Mitsuru, Nenoi en Akira, Fujimori en Bing, Wang
アクセス権	metadata only access
内容注記	Abstract Previous work pointed to a critical role of excessive production of reactive oxygen species (ROS) in increased radiation hematopoietic death in GFP mice. Meanwhile, enhanced antioxidant capability was not demonstrated in the mouse model of radio-induced adaptive response (RAR) using rescue of radiation hematopoietic death as the endpoint. ROS induction by ex vivo X-irradiation at a dose ranging from 0.1 to 7.5 Gy in the nucleated bone marrow cells was comparatively studied using GFP and wild type (WT) mice. ROS induction was also investigated in the cells collected from mice receiving a priming dose (0.5 Gy) efficient for RAR induction in WT mice. Significantly elevated background and increased induction of ROS in the cells from GFP mice were observed compared to those from WT mice. Markedly lower background and decreased induction of ROS were observed in the cells collected from WT mice but not GFP mice, both receiving the priming dose. GFP overexpression could alter background and induction of ROS by X-irradiation in hematopoietic cells. The results provide a reasonable explanation to the previous study on the fate of cells and mice after X-irradiation and confirm enhanced antioxidant capability in RAR. Investigations involving GFP overexpression should be carefully interpreted.
出版者	MDPI
日付	Issued2021-06
言語	eng
資源タイプ	journal article
資源識別子	URI https://repo.qst.go.jp/records/84059
関連	PMID 34203224 DOI https://doi.org/10.3390/ijms22136929 URI https://www.mdpi.com/1422-0067/22/13/6929
収録誌情報	ISSN 1422-0067 International Journal of Molecular Sciences 巻22 号13 開始ページ6929
コンテンツ更新日時	2023-07-10