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タイトル
  • en l-Anserine Increases Muscle Differentiation and Muscle Contractility in Human Skeletal Muscle Cells
作成者
    • en Nagai, Akitoshi
    • en Ida, Masayuki
    • en Izumo, Takayuki
    • en Nakai, Masaaki
    • en Honda, Hiroyuki
    • en Shimizu, Kazunori
アクセス権 open access
権利情報
  • en This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Agricultural and Food Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-3G5N54M2HSKVZXFE355F].”
主題
  • Other l-anserine
  • Other three-dimensional skeletal muscle tissue
  • Other microdevice
  • Other contractile force
  • Other histamine receptor
内容注記
  • Abstract en l-Anserine, an imidazole peptide, has a variety of physiological activities, but its effects on skeletal muscle differentiation and muscle contractile force remain unknown. Thus, in this study, we investigated the effect of l-anserine on muscle differentiation and muscle contractile force in human skeletal muscle cells. In two-dimensional culture, 1 μM l-anserine significantly increased the myotube diameters (26.5 ± 1.71, 27.7 ± 1.08, and 28.8 ± 0.85 μm with 0, 0.1, and 1 μM l-anserine, respectively) and the expression levels of genes involved in muscle differentiation and the sarcomere structure. In three-dimensional culture, 1 μM l-anserine significantly increased the contractile force of engineered human skeletal muscle tissues cultured on a microdevice (1.99 ± 0.30, 2.17 ± 0.62, 2.66 ± 0.39, and 3.28 ± 0.85 μN with 0, 0.1, 0.5, and 1 μM l-anserine, respectively). l-Anserine also increased the myotube diameters and the proportion of myotubes with sarcomere structures in the cultured tissues. Furthermore, the histamine receptor 1 (H1R) antagonist attenuated the l-anserine-induced increase in the contractile force, suggesting the involvement of H1R in the mechanism of action of l-anserine. This study showed for the first time that l-anserine enhances muscle differentiation and muscle contractility via H1R.
出版者 en ACS Publications
日付
    Available2024-06-14 , Issued2023-06-14
言語
  • eng
資源タイプ journal article
出版タイプ AM
資源識別子 HDL http://hdl.handle.net/2237/0002007916 , URI https://nagoya.repo.nii.ac.jp/records/2007916
関連
  • isVersionOf DOI https://doi.org/10.1021/acs.jafc.3c01685
収録誌情報
    • PISSN 0021-8561
      • en Journal of Agricultural and Food Chemistry
      • 巻71 号23 開始ページ8952 終了ページ8958
ファイル
コンテンツ更新日時 2024-07-05