Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel.

Shindo  Hisakazu; Yasui  Kiyoshi; Yamamoto  Kazuo; Honma  Kiri; Yui  Katsuyuki; Kohno  Tomoko; Ma  Yuhua; Chua  Koon Jiew; Kubo  Yoshinao; Aihara  Hitoshi; Ito  Takashi; Nagayasu  Takeshi; Matsuyama  Toshifumi; Hayashi  Hideki

タイトル	Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel.
作成者	Shindo, Hisakazu Yasui, Kiyoshi NRID 1000050372777 Yamamoto, Kazuo NRID 1000070255123 Honma, Kiri NRID 1000070307940 Yui, Katsuyuki NRID 1000090274638 Kohno, Tomoko NRID 1000060284684 Ma, Yuhua Chua, Koon Jiew Kubo, Yoshinao NRID 1000030273527 Aihara, Hitoshi NRID 1000080587717 Ito, Takashi NRID 1000090306275 Nagayasu, Takeshi NRID 1000080284686 Matsuyama, Toshifumi NRID 1000030165922 Hayashi, Hideki NRID 1000010218589
権利情報	Copyright © 2011 Elsevier Ltd. All rights reserved.
主題	other Adult T-cell leukemia/lymphoma (ATLL) other C-Rel other IL-2 other IL-4 other Interferon regulatory factor (IRF)-4
内容注記	Other Interferon regulatory factor (IRF)-4 is a member of the IRF transcription factor family, whose expression is primarily restricted to lymphoid and myeloid cells. In T-cells, IRF-4 expression is induced by T-cell receptor (TCR) cross-linking or treatment with phorbol-12-myristate-13-acetate (PMA)/Ionomycin, and IRF-4 is thought to be a critical factor for various functions of T-cells. To elucidate the IRF-4 functions in human adult T-cell leukemia virus type 1 (HTLV-1)-infected T-cells, which constitutively express IRF-4, we isolated IRF-4-binding proteins from T-cells, using a tandem affinity purification (TAP)-mass spectrometry strategy. Fourteen proteins were identified in the IRF-4-binding complex, including endogenous IRF-4 and the nuclear factor-kappaB (NF-κB) family member, c-Rel. The specific association of IRF-4 with c-Rel was confirmed by immunoprecipitation experiments, and IRF-4 was shown to enhance the c-Rel-dependent binding and activation of the interleukin-4 (IL-4) promoter region. We also demonstrated that IL-2 production was also enhanced by exogenously-expressed IRF-4 and c-Rel in the presence of P/I, in T-cells, and that the optimal IL-2 and IL-4 productions in vivo was IRF-4-dependent using IRF-4-/- mice. These data provide molecular evidence to support the clinical observation that elevated expression of c-Rel and IRF-4 is associated with the prognosis in adult T-cell leukemia/lymphoma (ATLL) patients, and present possible targets for future gene therapy. Other identifier:Cytokine, 56(3), pp.564-572; 2011 Other identifier:10960023
出版者	Elsevier Ltd.
日付	Issued2011-12
言語	eng
資源識別のタイプ	journal article
出版タイプ	AM
資源識別子	URI http://hdl.handle.net/10069/27006
関連	isIdenticalTo PMID 21890374 isIdenticalTo DOI https://doi.org/10.1016/j.cyto.2011.08.014
収録誌情報	ISSN 1043-4666 Cytokine 56(3), 564-572
ファイル	http://naosite.lb.nagasaki-u.ac.jp/dspace/bitstream/10069/27006/1/Cytokine56_564.pdf (application/pdf)
コンテンツ更新日時	2014-06-23