一覧に戻る

タイトル
  • Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis
作成者
権利情報
  • © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
主題
  • other cathepsin B
  • other chikungunya viruse
  • other endocytosis
  • other macropinocytosis
  • other murine leukemia virus vector
内容注記
  • Other Chikungunya virus (CHIKV) is an enveloped virus that enters host cells and transits within the endosomes before starting its replication cycle, the precise mechanism of which is yet to be elucidated. Endocytosis and endosome acidification inhibitors inhibit infection by CHIKV,murine leukemia virus (MLV), or SARS-coronavirus, indicating that these viral entries into host cells occur through endosomes and require endosome acidification. Although endosomal cathepsin B protease is necessary for MLV, Ebola virus, and SARS-CoV infections, its role in CHIKV infection is unknown. Our results revealed that endocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in 293T cells but not in TE671 cells. In contrast, macropinocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in TE671 cells but not in 293T cells, suggesting that CHIKV host cell entry occurs via endocytosis or macropinocytosis, depending on the cell lines used. Cathepsin B inhibitor and knockdown by an shRNA suppressed CHIKV-pseudotyped MLV vector infection both in 293T and TE671 cells. These results show that cathepsin B facilitates CHIKV infection regardless of the entry pathway.
  • Other identifier:Viruses, 12(7), art.no.722; 2020
  • Other identifier:19994915
出版者 MDPI
日付
    Issued2020-07-03
言語
  • eng
資源識別のタイプ journal article
出版タイプ VoR
資源識別子 URI http://hdl.handle.net/10069/40150
関連
  • isIdenticalTo DOI https://doi.org/10.3390/v12070722
収録誌情報
  • Viruses 12(7), 722
ファイル
コンテンツ更新日時 2020-07-22