| タイトル |
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Rhoptry neck protein 4 plays important roles during Plasmodium sporozoite infection of the mammalian liver
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| 作成者 |
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| 権利情報 |
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en
ⓒ 2023 Baba et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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| 主題 |
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Other
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malaria
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Other
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Plasmodium
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Other
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sporozoite
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Other
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rhoptry
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Other
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invasion
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| 内容注記 |
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Abstract
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During invasion, Plasmodium parasites secrete proteins from rhoptry and microneme apical end organelles, which have crucial roles in attaching to and invading target cells. A sporozoite stage-specific gene silencing system revealed that rhoptry neck protein 2 (RON2), RON4, and RON5 are important for sporozoite invasion of mosquito salivary glands. Here, we further investigated the roles of RON4 during sporozoite infection of the liver in vivo. Following intravenous inoculation of RON4-knockdown sporozoites into mice, we demonstrated that sporozoite RON4 has multiple functions during sporozoite traversal of sinusoidal cells and infection of hepatocytes. In vitro infection experiments using a hepatoma cell line revealed that secreted RON4 is involved in sporozoite adhesion to hepatocytes and has an important role in the early steps of hepatocyte infection. In addition, in vitro motility assays indicated that RON4 is required for sporozoite attachment to the substrate and the onset of migration. These findingsfindingsindicate that RON4 is crucial for sporozoite migration toward and invasion of hepatocytes via attachment ability and motility.
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Other
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mSphere, 8(4), art. no. e0058722; 2023
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| 出版者 |
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American Society for Microbiology
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/10069/0002000161
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URI
https://nagasaki-u.repo.nii.ac.jp/records/2000161
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.1128/msphere.00587-22
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| 収録誌情報 |
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mSphere
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巻8
号4
開始ページart. no. e0058722
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| ファイル |
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| コンテンツ更新日時 |
2023-09-07 |
