| タイトル |
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en
Synthesis of a new NIR fluorescent Nd complex labeling agent.
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| 作成者 |
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en
Shimizu, Yoichi
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e-Rad_Researcher 90634212
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en
Saji, Hideo
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佐治, 英郎
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e-Rad_Researcher 40115853
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| アクセス権 |
open access |
| 権利情報 |
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en
The original publication is available at www.springerlink.com
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en
This is not the published version. Please cite only the published version.
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ja
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
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| 主題 |
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Other
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Neodymium
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Other
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Near-infrared
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Other
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Fluorescent labeling
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Other
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Maleimide
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MeSH
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Animals
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MeSH
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Avidin/metabolism
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MeSH
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Biological Transport
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MeSH
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Biotin/metabolism
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MeSH
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Cell Line, Tumor
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MeSH
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Fluorescent Dyes/chemical synthesis
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MeSH
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Fluorescent Dyes/chemistry
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MeSH
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Fluorescent Dyes/metabolism
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MeSH
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Heterocyclic Compounds, 1-Ring/chemistry
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MeSH
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Infrared Rays
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MeSH
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Maleimides/chemistry
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MeSH
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Neodymium/chemistry
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MeSH
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Organometallic Compounds/chemical synthesis
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MeSH
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Organometallic Compounds/chemistry
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MeSH
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Organometallic Compounds/metabolism
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MeSH
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Rats
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MeSH
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Spectrometry, Fluorescence
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| 内容注記 |
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Abstract
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Fluorescent analysis has been widely used in biological, chemical and analytical research. A useful fluorescent labeling agent should include NIR emission, a large Stoke's shift, and good labeling ability without interfering with the pharmacological profile of the labeled compound. Thus, we planned to develop an M-AMF-DOTA(Nd) derivative composed of an NIR fluorescent moiety and a maleimide conjugating moiety as a new NIR fluorescent labeling agent which fulfills these requirements. M-AMF-DOTA(Nd) was synthesized from 4-amino-fluorescein and was conjugated with an avidin molecule (Avidin-AMF-DOTA(Nd)) through Lys-side chains by reaction with 2-iminothiolane. The fluorescent features of M-AMF-DOTA(Nd) and Avidin-AMF-DOTA(Nd) were comparatively evaluated. A binding assay of Avidin-AMF-DOTA(Nd) with D-biotin and a tumor cell-uptake study were performed to estimate the effects of conjugation on the biological and physicochemical features of the protein. M-AMF-DOTA(Nd) was obtained in 22% overall yield. M-AMF-DOTA(Nd) had a typical NIR fluorescence from the Nd ion (880 nm and 900 nm from 488 nm excitation). Avidin-AMF-DOTA(Nd) was easily synthesized and also had typical NIR fluorescence from the Nd ion without loss of fluorescent intensity. The binding affinity of Avidin-AMF-DOTA(Nd) to D-biotin was equivalent to naive avidin. Avidin-AMF-DOTA(Nd) was taken up by tumor cells in the same manner as avidin conjugated with fluorescein isothiocyanate, an established, widely used fluorescent avidin. Results from this study indicate that M-AMF-DOTA(Nd) is a potential labeling agent for routine NIR fluorescent analysis.
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| 出版者 |
en
Springer
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2433/128929
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.1007/s10895-009-0542-3
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isIdenticalTo
PMID
19821013
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| 収録誌情報 |
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PISSN
1053-0509
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EISSN
1573-4994
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NCID
AA10871550
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Journal of fluorescence
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巻20
号1
開始ページ225
終了ページ234
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| ファイル |
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| コンテンツ更新日時 |
2026-02-18 |
