Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells

Satoru Mizuhashi; Satoshi Fukushima; Takayuki Ishibashi; Haruka Kuriyama; Toshihiro Kimura; Hisashi Kanemaru; Ikko Kajihara; Katsunari Makino; Azusa Miyashita; Jun Aoi; Kanako Kita; Hironobu Ihn

タイトル	en Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells
作成者	en Satoru, Mizuhashi en Satoshi, Fukushima en Takayuki, Ishibashi en Haruka, Kuriyama en Toshihiro, Kimura en Hisashi, Kanemaru en Ikko, Kajihara en Katsunari, Makino en Azusa, Miyashita en Jun, Aoi en Kanako, Kita en Hironobu, Ihn
権利情報	en (C) 2021 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
主題	Other en Melanoma Other en Nucleosome assembly protein 1-like4 Other en Migration Other en Invasion Other en Slug Other en p21
内容注記	Abstract en Background: Melanoma is one of the deadliest skin cancers. The treatment of advanced melanoma has been dramatically improved by immune checkpoint inhibitors and targeted therapies. However, many patients still do not respond to these therapies. Objective: To investigate whether NAP1L4 can be a new therapeutic target for melanoma. Methods: Immunohistochemical analysis of human nevus and melanoma tissues was performed. Real-time RT-PCR and immunoblotting were performed using human samples and melanoma cell lines. Next, we examined the effect of NAP1L4 knockdown in melanoma cell lines using cell migration and invasion assays. To investigate the molecular mechanism related to these results, immunoblotting of p21 and Slug was examined. MMP-2 and MMP-9 activity assays were also performed. Further, pathway analysis between NAP1L4 and MMP-2 was performed. Finally, the effects of NAP1L4 knockdown on cell proliferation, apoptosis, and cell cycle were analyzed. Results: NAP1L4 was overexpressed in melanoma tissues compared to the nevus tissue. NAP1L4 knockdown reduced melanoma cell migration and invasion. NAP1L4 knockdown upregulated p21 and downregulated Slug expression in melanoma cells. NAP1L4 knockdown decreased the active levels of MMP-2 in the supernatant from melanoma cells. NAP1L4 knockdown inhibited apoptosis in camptothecin-induced DNA damage, induced cell cycle arrest at the G1/S phase, and inhibited cell proliferation. Conclusions: NAP1L4 may play a role in cell migration and invasion in melanoma cells through the regulation of Slug. We propose that NAP1L4 can be a new therapeutic target for proliferation and invasion of melanoma cells.
出版者	en Elsevier
日付	Issued2021-04-01
言語	eng
資源タイプ	journal article
出版タイプ	AM
資源識別子	HDL http://hdl.handle.net/2298/0002000012 , URI https://kumadai.repo.nii.ac.jp/records/2000012
関連	isVersionOf DOI https://doi.org/10.1016/j.jdermsci.2021.02.001
収録誌情報	PISSN 0923-1811 en Journal of Dermatological Science 巻102 号1 開始ページ16 終了ページ24
ファイル	https://kumadai.repo.nii.ac.jp/record/2000012/files/j_jdermsci_202102001.pdf 1.1 MB (application/pdf) Available2023-09-19
コンテンツ更新日時	2023-10-13