GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

Nishimura Hideki; 西村 英樹; ニシムラ ヒデキ

タイトル	en GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells
作成者	en Nishimura, Hideki ja 西村, 英樹 ja-Kana ニシムラ, ヒデキ
アクセス権	open access
権利情報	en Copyright © Spandidos Publications 2016.
主題	Other en glycogen synthase kinase-3 Other en SB216763 Other en apoptosis Other en osteosarcoma
内容注記	Abstract en Abstract Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. The functions of GSK-3 in cancer differ depending on cell type. In the present study, we examined the effects of a specific GSK-3 inhibitor on the regulation of osteosarcoma cell proliferation and apoptosis. Immunohistochemical analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to determine the expression pattern of GSK-3 in human osteosarcoma cells. We used the MTS assay, western blotting, measurement of single-stranded DNA and morphological analyses to study the effects of a GSK-3 inhibitor, SB216763 on osteosarcoma cell proliferation and survival. We detected an increase in mRNA expression of GSK-3 and aberrant nuclear accumulation of GSK-3 in the osteosarcoma cells. Pharmacological inhibition of GSK-3 led to a decrease in proliferation and survival of osteosarcoma cells. Inhibition of GSK-3 resulted in a decreased expression of Bcl-2 and a subsequent increase in osteosarcoma cell apoptosis via the mitochondrial pathway. The present study demonstrated that GSK-3 activity is critical for tumorigenicity and cell survival in osteosarcoma cells. Our findings suggest that GSK-3 is a potential therapeutic target for the treatment of human osteosarcoma.
言語	eng
資源タイプ	doctoral thesis
出版タイプ	P
資源識別子	URI https://kagawa-u.repo.nii.ac.jp/records/376
関連	isVersionOf DOI https://doi.org/10.3892/or.2016.4565 isIdenticalTo URI https://dl.ndl.go.jp/pid/11337526 PMID 26781995 NAID 500001356509
学位情報	学位授与番号甲第622号学位授与機関識別子名 kakenhi 識別子 16201 機関名称 ja 香川大学 en Kagawa University 学位授与年月日 2016-03-24 学位名 ja 博士（医学）
ファイル	fulltext 本文 1.9 MB (application/pdf) Available2020-11-06 abstract 内容の要旨 64.3 kB (application/pdf) Available2020-11-06 other 審査の結果の要旨 509.3 kB (application/pdf) Available2020-11-06
コンテンツ更新日時	2024-02-10