| タイトル |
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en
Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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en
© 2006 by The American Society for Cell Biology
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| 主題 |
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| 内容注記 |
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Abstract
en
The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2–mediated sex determination pathway is crucial for the rpn-10–induced sterile phenotype. Intriguingly, we found that co-knockdown of Δrpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10–mediated ubiquitin pathway is indispensable for control of the TRA-2–mediated sex-determining pathway.
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| 出版者 |
en
The American Society for Cell Biology
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/17253
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| 関連 |
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URI
http://www.ascb.org/
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isIdenticalTo
DOI
https://doi.org/10.1091/mbc.E06-05-0437
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PMID
17050737
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| 収録誌情報 |
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en
Molecular Biology of the Cell
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巻17
号12
開始ページ5356
終了ページ5371
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
