| タイトル |
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en
High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.
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| 作成者 |
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| アクセス権 |
open access |
| 主題 |
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Other
en
cell density
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Other
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mesenchymal stem cell
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Other
en
differentiation
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Other
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chondrocyte
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NDC
463
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| 内容注記 |
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Abstract
en
The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.
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| 出版者 |
en
The Society for Biotechnology, Japan
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/22086
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| 関連 |
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URI
http://www.sbj.or.jp/
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isVersionOf
DOI
https://doi.org/10.1263/jbb.103.98
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PMID
17298908
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| 収録誌情報 |
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PISSN
1389-1723
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EISSN
1347-4421
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en
Journal of Bioscience and Bioengineering
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巻103
号1
開始ページ98
終了ページ100
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
