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タイトル
  • en Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum
作成者
アクセス権 open access
権利情報
  • en The original publication is available at www.springerlink.com
主題
  • Other en Fish serum
  • Other en Fetal calf serum
  • Other en Chinese hamster ovary cells
  • NDC 463
内容注記
  • Abstract en The effects of fish serum on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of Chinese hamster ovary (CHO) cells DR1000L4N were investigated and compared with those of fetal calf serum (FCS). Although fish serum did not stimulate the initial adhesion of CHO cells to culture dishes, it prompted cell growth after cell adhesion with FCS for 24 h. The cell density in the fish serum medium reached 75% that in the FCS medium. Fish serum promoted cell adhesion to and cell growth on collagen-coated dishes. The cell-specific production rate of hGM-CSF in the fish serum medium on collagen-coated dishes was almost the same as that in the FCS medium.
出版者 en Springer
日付
    Issued2007-07
言語
  • eng
資源タイプ journal article
出版タイプ AM
資源識別子 HDL http://hdl.handle.net/2115/28048
関連
  • isVersionOf DOI https://doi.org/10.1007/s00253-007-0897-9
  • PMID 17334756
収録誌情報
    • PISSN 0175-7598
    • EISSN 1432-0614
      • en Applied Microbiology and Biotechnology
      • 75 5 開始ページ983 終了ページ987
ファイル
    • fulltext AMB75-5.pdf
    • 164.97 KB (application/pdf)
      • Issued2007-07
コンテンツ更新日時 2023-07-26