タイトル |
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en
Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum
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作成者 |
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アクセス権 |
open access |
権利情報 |
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en
The original publication is available at www.springerlink.com
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主題 |
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Other
en
Fish serum
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Other
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Fetal calf serum
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Other
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Chinese hamster ovary cells
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NDC
463
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内容注記 |
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Abstract
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The effects of fish serum on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of Chinese hamster ovary (CHO) cells DR1000L4N were investigated and compared with those of fetal calf serum (FCS). Although fish serum did not stimulate the initial adhesion of CHO cells to culture dishes, it prompted cell growth after cell adhesion with FCS for 24 h. The cell density in the fish serum medium reached 75% that in the FCS medium. Fish serum promoted cell adhesion to and cell growth on collagen-coated dishes. The cell-specific production rate of hGM-CSF in the fish serum medium on collagen-coated dishes was almost the same as that in the FCS medium.
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出版者 |
en
Springer
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日付 |
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言語 |
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資源タイプ |
journal article |
出版タイプ |
AM |
資源識別子 |
HDL
http://hdl.handle.net/2115/28048
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関連 |
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isVersionOf
DOI
https://doi.org/10.1007/s00253-007-0897-9
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PMID
17334756
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収録誌情報 |
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PISSN
0175-7598
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EISSN
1432-0614
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en
Applied Microbiology and Biotechnology
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巻75
号5
開始ページ983
終了ページ987
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ファイル |
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コンテンツ更新日時 |
2023-07-26 |