| タイトル |
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en
Functional Importance of the Coiled-Coil of the Ebola Virus Glycoprotein
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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en
Copyright © 2000 American Society for Microbiology
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| 主題 |
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| 内容注記 |
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Abstract
en
Ebola virus contains a single glycoprotein (GP) that is responsible for receptor binding and membrane fusion and is proteolytically cleaved into disulfide-linked GP1 and GP2 subunits. The GP2 subunit possesses a coiled-coil motif, which plays an important role in the oligomerization and fusion activity of other viral GPs. To determine the functional significance of the coiled-coil motif of GP2, we examined the effects of peptides corresponding to the coiled-coil motif of GP2 on the infectivity of a mutant vesicular stomatitis virus (lacking the receptor-binding/fusion protein) pseudotyped with the Ebola virus GP. A peptide corresponding to the C-terminal helix reduced the infectivity of the pseudotyped virus. We next introduced alanine substitutions into hydrophobic residues in the coiled-coil motif to identify residues important for GP function. None of the substitutions affected GP oligomerization, but some mutations, two in the N-terminal helix and all in the C-terminal helix, reduced the ability of GP to confer infectivity to the mutant vesicular stomatitis virus without affecting the transport of GP to the cell surface, its incorporation into virions, and the production of virus particles. These results indicate that the coiled-coil motif of GP2 plays an important role in facilitating the entry of Ebola virus into host cells and that peptides corresponding to this region could act as efficient antiviral agents.
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| 出版者 |
en
American Society for Microbiology
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/28145
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.1128/JVI.74.21.10194-10201.2000
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PMID
11024148
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| 収録誌情報 |
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en
Journal of Virology
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巻74
号21
開始ページ10194
終了ページ10201
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
