Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the α2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks

Ito Toshihiro; Suzuki Yasuo; Suzuki Takashi; Takada Ayato; Horimoto Taisuke; Wells Krisna; Kida Hiroshi; Otsuki Koichi; Kiso Makoto; Ishida Hideharu; Kawaoka Yoshihiro

タイトル	en Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the α2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks
作成者	en Ito, Toshihiro en Suzuki, Yasuo en Suzuki, Takashi NRID 1000030196836 en Takada, Ayato NRID 1000010292062 en Horimoto, Taisuke NRID 1000000222282 en Wells, Krisna en Kida, Hiroshi NRID 1000010109506 en Otsuki, Koichi en Kiso, Makoto NRID 1000090092931 en Ishida, Hideharu NRID 1000020203002 en Kawaoka, Yoshihiro NRID 1000070135838
アクセス権	open access
権利情報	en Copyright © 2000 American Society for Microbiology
主題	NDC 649
内容注記	Abstract en The hemagglutinin (HA) of H3 human influenza viruses does not support viral replication in duck intestine despite its avian origin. A Leu-to-Gln mutation at position 226 and a Ser-to-Gly mutation at position 228 in the HA of human A/Udorn/307/72 (H3N2) permit a reassortant virus [human Udorn HA, with all other genes from A/mallard/New York/6750/78 (H2N2)] to replicate in ducks. To understand the molecular basis of this change in host range restriction, we investigated the receptor specificity of duck influenza viruses as well as of human-duck virus reassortants. The results indicate that the recognition of a glycoconjugate moiety possessing N-glycolneuramic acid (NeuGc) linked to galactose by the α2,3 linkage (NeuGcα2,3Gal) is associated with viral replication in duck intestine. Immunofluorescence assays with NeuGcα2,3Gal-specific antiserum detected this moiety primarily on the crypt epithelial cells of duck colon. Such recognition, together with biochemical evidence of NeuGc in crypt cells, correlated exactly with the ability of the virus to replicate in duck colon. These results suggest that recognition of the NeuGcα2,3-Gal moiety plays an important role in the enterotropism of avian influenza viruses.
出版者	en American Society for Microbiology
日付	Issued2000-10
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/28146
関連	isIdenticalTo DOI https://doi.org/10.1128/JVI.74.19.9300-9305.2000 PMID 10982377
収録誌情報	PISSN 0022-538X en Journal of Virology 巻74 号19 開始ページ9300 終了ページ9305
ファイル	fulltext JV74-19.pdf 1.04 MB (application/pdf) Issued2000-10
コンテンツ更新日時	2023-07-26