Identification of a Novel Vinyl Reductase Gene Essential for the Biosynthesis of Monovinyl Chlorophyll in Synechocystis sp. PCC6803

Ito Hisashi; Yokono Makio; Tanaka Ryouichi; Tanaka Ayumi

タイトル	en Identification of a Novel Vinyl Reductase Gene Essential for the Biosynthesis of Monovinyl Chlorophyll in Synechocystis sp. PCC6803
作成者	en Ito, Hisashi en Yokono, Makio en Tanaka, Ryouichi NRID 1000020311516 en Tanaka, Ayumi NRID 1000010197402
アクセス権	open access
権利情報	en Copyright © 2008 the American Society for Biochemistry and Molecular Biology
主題	NDC 464
内容注記	Abstract en The vast majority of oxygenic photosynthetic organisms use monovinyl chlorophyll for their photosynthetic reactions. For the biosynthesis of this type of chlorophyll, the reduction of the 8-vinyl group that is located on the B ring of the macrocycle is essential. Previously, we identified the gene encoding 8-vinyl reductase responsible for this reaction in higher plants, and termed it DVR. Among the sequenced genomes of cyanobacteria, only several Synechococcus species contain DVR homologues. Therefore, it has been hypothesized that many other cyanobacteria producing monovinyl chlorophyll should contain a vinyl reductase which is unrelated to the higher-plant DVR. In order to identify the cyanobacterial gene that is responsible for monovinyl chlorophyll synthesis, we developed a bioinformatics tool, CCCT (Correlation Coefficient Calculation Tool), which calculates the correlation coefficient between the distributions of a certain phenotype and genes among a group of organisms. The program indicated that the distribution of a gene encoding a putative dehydrogenase-protein is best correlated with the distribution of the DVR-less cyanobacteria. We subsequently knocked out the corresponding gene (Slr1923) in Synechocystis sp. PCC6803 and characterized the mutant. The knock-out mutant lost its ability to synthesize monovinyl chlorophyll and, accumulated 3,8-divinyl chlorophyll instead. We concluded that Slr1923 encodes the vinyl reductase or a subunit essential for monovinyl chlorophyll synthesis. The function and evolution of 8-vinyl reductase genes are discussed.
出版者	en The American Society for Biochemistry and Molecular Biology
日付	Issued2008-04-04
言語	eng
資源タイプ	journal article
出版タイプ	AM
資源識別子	HDL http://hdl.handle.net/2115/33904
関連	isVersionOf DOI https://doi.org/10.1074/jbc.M708369200 PMID 18230620
収録誌情報	PISSN 0021-9258 EISSN 1083-351X NCID AA00251083 en Journal of Biological Chemistry 巻283 号14 開始ページ9002 終了ページ9011
ファイル	fulltext tanaka.pdf 940.26 KB (application/pdf) Issued2008-04-04
コンテンツ更新日時	2023-07-26