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Involvement of chondroitin sulfate E in the liver tumor focal formation of murine osteosarcoma cells
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| 作成者 |
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| 権利情報 |
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This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The definitive publisher-authenticated version 19(7):735-742, July 2009 is available online at: http://dx.doi.org/10.1093/glycob/cwp041
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| 主題 |
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Other
chondroitin sulfate
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| 内容注記 |
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Cell surface heparan sulfate plays a critical role in regulating the metastatic behavior of tumor cells, whereas the role of chondroitin sulfate/dermatan sulfate (CS/DS) has been little understood in this context. Here, we characterized CS/DS chains from the murine osteosarcoma cell line LM8G7, which forms tumor nodules in liver. Structural analysis of the CS/DS chains showed a higher proportion of GlcUAβ1-3GalNAc(4,6-O-disulfate) (E-units) in LM8G7 (12%) than in its parental cell line LM8 (6%), which rarely forms tumors in the liver. Immunostaining with GD3G7, an antibody specific to E-units, confirmed the higher expression of the epitope in LM8G7 than LM8 cells. The tumor focal formation of LM8G7 cells in the liver in mice was effectively inhibited by the pre-administration of CS-E (rich in E-unit) or the pre-incubation of the antibody GD3G7 with the tumor cells. CS-E or GD3G7 inhibited the adhesion of LM8G7 cells to a laminin-coated plate in vitro. In addition, the invasive ability of LM8G7 cells in vitro was also reduced by the addition of CS-E or the antibody. Further, CS-E or the antibody inhibited the proliferation of LM8G7 cells dose-dependently. The binding of LM8G7 cells to VEGF in vitro was also significantly reduced by CS-E and GD3G7. Thus, the present study reveals the significance of highly sulfated CS/DS structures in the liver colonization of osteosarcoma cells and also provides a framework for the development of GAG-based anti-cancer molecules.
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| 出版者 | Oxford University Press
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| 日付 | Issued 2009-07
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| 言語 | eng |
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| 資源タイプ | journal article |
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| 出版タイプ | AM |
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| 資源識別子 | URI http://hdl.handle.net/2115/43174
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| 関連 |
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PMID
19293233
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DOI
https://doi.org/10.1093/glycob/cwp041
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| コンテンツ更新日時 | 2019-03-15T05:20:33Z |
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