| タイトル |
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en
Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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en
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The definitive publisher-authenticated version 19(5):488-498, May 2009 is available online at: http://dx.doi.org/10.1093/glycob/cwp005
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| 主題 |
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Other
en
Xenopus laevis
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Other
en
chondroitin sulfate
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Other
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heparan sulfate
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Other
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glycosaminoglycan
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Other
en
embryogenesis
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NDC
491
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| 内容注記 |
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Abstract
en
Xenopus laevis is an excellent animal for analyzing early vertebrate development. Various effects of glycosaminoglycans (GAGs) on growth factor-related cellular events during embryogenesis have been demonstrated in Xenopus. To elucidate the relationship between alterations in fine structure and changes in the specificity of growth factor-binding during Xenopus development, heparan sulfate (HS) and chondroitin/dermatan sulfate (CS/DS) chains were isolated at four different embryonic stages and their structure and growth factor-binding capacities were compared. The total amounts of both HS and CS/DS chains decreased from the pre-midblastula transition to the gastrula stage, but increased exponentially during the following developmental stages. The length of HS chains was not significantly affected by development, whereas that of CS/DS chains increased with development. The disaccharide composition of GAGs in embryos also changed during development. The degree of sulfation of the HS chains gradually decreased with development. The predominant sulfation position in the CS/DS chains shifted from C4 to C6 of GalNAc during embryogenesis. Growth factor-binding experiments using a BIAcore system demonstrated that GAGs bound growth factors including fibroblast growth factors-1, and -2, midkine, and pleiotrophin, with comparable affinities. These affinities significantly varied during development, although the correlation between the structural alterations of GAGs and the change in the ability to bind growth factors remains to be clarified. Expression of saccharide sequences, which specifically interact with a growth factor, might be regulated during development.
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| 出版者 |
en
Oxford University Press
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/43036
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.1093/glycob/cwp005
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PMID
19190026
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| 収録誌情報 |
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PISSN
0959-6658
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EISSN
1460-2423
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en
Glycobiology
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巻19
号5
開始ページ488
終了ページ498
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
