Prevalence and Carrier Status of Leptospirosis in Smallholder Dairy Cattle and Peridomestic Rodents in Kandy, Sri Lanka

Gamage Chandika D.; Koizumi Nobuo; Muto Maki; Nwafor-Okoli Chinyere; Kurukurusuriya Shanika; Rajapakse Jayanthe R. P. V.; Kularatne Senanayake A. M.; Kanda Koji; Lee Romeo B.; Obayashi Yoshihide; Watanabe Haruo; Tamashiro Hiko

タイトル	en Prevalence and Carrier Status of Leptospirosis in Smallholder Dairy Cattle and Peridomestic Rodents in Kandy, Sri Lanka
作成者	en Gamage, Chandika D. en Koizumi, Nobuo en Muto, Maki en Nwafor-Okoli, Chinyere en Kurukurusuriya, Shanika en Rajapakse, Jayanthe R. P. V. en Kularatne, Senanayake A. M. en Kanda, Koji en Lee, Romeo B. en Obayashi, Yoshihide en Watanabe, Haruo en Tamashiro, Hiko NRID 1000060322799
アクセス権	open access
権利情報	en This is a copy of an article published in the Vector-Borne and Zoonotic Diseases © 2011 copyright Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at: http://www.liebertonline.com.
主題	Other en Domestic Animals Other en Leptospirosis Other en PCR Other en Serology Other en Zoonosis NDC 465
内容注記	Abstract en Leptospirosis is an important bacterial zoonotic disease globally and one of the notifiable diseases in Sri Lanka. Other than human leptospirosis, little information is available on leptospirosis in domestic and feral animals in Sri Lanka. Thus, this study attempted to determine the prevalence and carrier status of leptospirosis in small-holder dairy cattle and peridomestic rodents to understand the impact of the disease on public health in Kandy, Sri Lanka. Cattle and rodent samples were collected from the Yatinuwara and Udunuwara divisional secretaries in Kandy. Serum samples were analyzed for the presence of antileptospiral antibodies using microscopic agglutination test. DNA was extracted from cattle urine and rodent kidney tissue samples, in which polymerase chain reaction was carried out to detect the Leptospira flaB gene. The cattle in 19 (38.8%) of the 49 farms harbored antileptospiral antibodies. Out of 113 cattle serum samples, 23 (20.3%) were positive; 17 (73.9%) and 6 (26.1%) reacted with serogroups Sejroe and Hebdomadis, respectively. Out of the 74 rodent samples, 13 (17.5%) were positive; 8 (61.5%) and 4 (30.8%) had reactions to serogroups Javanica and Icterohaemorrhagiae, respectively. Leptospiral DNA was detected in one cattle urine sample and identified as Leptospira interrogans. This study revealed a high prevalence of leptospirosis in cattle and rodents in Kandy. These animals were infected with a wide array of leptospiral serogroups, which are consistent with the research findings observed in humans in Kandy. Overall, serological data indicate that relative to rodents, cattle may be a more significant reservoir for human transmission and a greater source of potential risk to local agricultural communities.
出版者	en Mary Ann Liebert
日付	Issued2011-08-08
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/47130
関連	isIdenticalTo DOI https://doi.org/10.1089/vbz.2010.0153 PMID 21284522
収録誌情報	PISSN 1530-3667 en Vector-Borne and Zoonotic Diseases 巻11 号8 開始ページ1041 終了ページ1047
ファイル	fulltext VBZD11-8_1041-1047.pdf 248.21 KB (application/pdf) Issued2011-08-08
コンテンツ更新日時	2023-07-26