タイトル |
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Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3
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作成者 |
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アクセス権 |
open access |
権利情報 |
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en
The original publication is available at www.springerlink.com
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主題 |
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Other
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Bombyx mori
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Other
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Sericin-1
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Other
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Silk gene
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Other
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Silk gland
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Other
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Homeodomain
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Other
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Gene gun
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NDC
467
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内容注記 |
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Abstract
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The sericin-1 gene encoding a glue protein is expressed in the middle silk gland (MSG) of the silkworm, Bombyx mori. A member of the class III POU domain transcription factors, POU-M1, was cloned as the factor bound to the SC site of the sericin-1 promoter and has been proposed to be a positive transcription factor. In this study, we analyzed the expression pattern of the POU-M1 gene in fourth and fifth instars in comparison with the pattern of the sericin-1 gene. The POU-M1 gene was expressed strongly in the region anterior to the sericin-1-expressing portion of the silk gland at both feeding stages. As the sericin-1-expressing region expands from the posterior to middle portions of the MSG in the fifth instar, the POU-M1-expressing region retreated from the middle to anterior portion. Introduction of the expression vector of POU-M1 into the silk glands by gene gun technology repressed promoter activity of the sericin-1 gene, suggesting that POU-M1 regulates the sericin-1 gene negatively. An in vitro binding assay showed that POU-M1 bound not only to the SC site but also to other promoter elements newly detected in vivo. Another spatiotemporal specific factor MIC binds to these elements, and POU-M1 competed with MIC to bind at the -70 site essential for promoter activity. These results suggest that POU-M1 is involved in restricting the anterior boundary of the sericin-1-expressing region in the silk gland by inhibiting the binding of the transcriptional activator to the promoter elements.
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出版者 |
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Springer-Verlag
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日付 |
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言語 |
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資源タイプ |
journal article |
出版タイプ |
AM |
資源識別子 |
HDL
http://hdl.handle.net/2115/53467
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関連 |
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isVersionOf
DOI
https://doi.org/10.1007/s00427-012-0418-4
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PMID
23070540
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収録誌情報 |
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Development Genes and Evolution
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巻222
号6
開始ページ351
終了ページ359
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ファイル |
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コンテンツ更新日時 |
2023-07-26 |