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タイトル
  • en A pH-sensitive cationic lipid facilitates the delivery of liposomal siRNA and gene silencing activity in vitro and in vivo
作成者
アクセス権 open access
主題
  • Other en Multifunctional envelope-type nano device (MEND)
  • Other en siRNA delivery
  • Other en pH-sensitive cationic lipid
  • Other en Intracellular trafficking
  • Other en Endosomal escape
  • NDC 499
内容注記
  • Abstract en Modification of liposomal siRNA carriers with polyethylene glycol, i.e., PEGylation, is a generally accepted strategy for achieving in vivo stability and delivery to tumor tissue. However, PEGylation significantly inhibits both cellular uptake and the endosomal escape process of the carriers. In a previous study, we reported on the development of a multifunctional envelope-type nano device (MEND) for siRNA delivery and peptide-based functional devices for overcoming the limitations and succeeded in the efficient delivery of siRNA to tumors. In this study, we synthesized a pH-sensitive cationic lipid, YSK05, to overcome the limitations. The YSK05-MEND had a higher ability for endosomal escape than other MENDs containing conventional cationic lipids. The PEGylated YSK05-MEND induced efficient gene silencing and overcame the limitations followed by optimization of the lipid composition. Furthermore, the intratumoral administration of the YSK05-MEND resulted in a more efficient gene silencing compared with MENDs containing conventional cationic lipids. Collectively, these data confirm that YSK05 facilitates the endosomal escape of the MEND and thereby enhances the efficacy of siRNA delivery into cytosol and gene silencing.
出版者 en Elsevier B.V.
日付
    Issued2012-11-10
言語
  • eng
資源タイプ journal article
出版タイプ AM
資源識別子 HDL http://hdl.handle.net/2115/51165
関連
  • isVersionOf DOI https://doi.org/10.1016/j.jconrel.2012.09.009
  • PMID 23000694
収録誌情報
    • PISSN 0168-3659
      • en Journal of Controlled Release
      • 163 3 開始ページ267 終了ページ276
ファイル
コンテンツ更新日時 2023-07-26