| タイトル |
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en
Identification of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater
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| 作成者 |
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| アクセス権 |
open access |
| 主題 |
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Other
en
Membrane bioreactor
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Other
en
Membrane fouling
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Other
en
Foulant characterization
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Other
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Proteins
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NDC
519
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| 内容注記 |
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Abstract
en
The proteins that caused membrane fouling in a continuous operation of MBRs treating real municipal wastewater were investigated in detail. We continuously operated two identical pilot-scale MBRs under different solid retention times (SRTs) and extracted the foulants at the end of the operation. Regardless of the operating conditions, proteins were dominant components in the foulants extracted from the fouled membranes. The extracted proteins were subjected to the separation with two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and the identification through the N-terminal amino acid sequencing analysis. The proteins concentrated by the combination of the crude concentration using an ultra-filtration (UF) membrane and trichloroacetic acid (TCA) precipitation were separated and visualized well on 2D-PAGE gels. The results of 2D-PAGE analysis indicated that the compositions of proteins that caused membrane fouling significantly differed depending on the SRT, although such differences cannot be seen in the amino acid composition analysis. Analyzing selected 2D-PAGE spots by N-terminal amino acid sequencing analysis led to the identification of two well-characterized outer membrane proteins originating from Pseudomonas genus, namely OprF and OprD. To our knowledge, this is the first successful identification of proteins that have caused membrane fouling in continuous operations of MBRs treating real wastewater.
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| 出版者 |
en
Elsevier
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/52031
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.1016/j.ibiod.2012.08.003
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| 収録誌情報 |
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en
International Biodeterioration & Biodegradation
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巻75
開始ページ15
終了ページ22
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
