Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice

Lennikov Anton; Kitaichi Nobuyoshi; Kase Satoru; Noda Kousuke; Horie Yukihiro; Nakai Akira; Ohno Shigeaki; Ishida Susumu

タイトル	en Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice
作成者	en Lennikov, Anton en Kitaichi, Nobuyoshi NRID 1000040431366 en Kase, Satoru NRID 1000060374394 en Noda, Kousuke NRID 1000090296666 en Horie, Yukihiro en Nakai, Akira en Ohno, Shigeaki NRID 1000050002382 en Ishida, Susumu NRID 1000010245558
アクセス権	open access
権利情報	http://creativecommons.org/licenses/by/3.0/ en Creative Commons Attribution 3.0 Unported
主題	Other en GGA Other en geranylgeranylacetone Other en HSP Other en HSP70 Other en UVB Other en keratitis Other en cornea Other en apoptosis NDC 496
内容注記	Abstract en Acute ultraviolet (UV) B exposure causes photokeratitis and induces apoptosis in corneal cells. Geranylgeranylacetone (GGA) is an acyclic polyisoprenoid that induces expression of heat shock protein (HSP) 70, a soluble intracellular chaperone protein expressed in various tissues, protecting cells against stress conditions. We examined whether induction of HSP70 has therapeutic effects on UV-photokeratitis in mice. C57 BL/6 mice were divided into four groups, GGA-treated (500 mg/kg/mouse) and UVB-exposed (400 mJ/cm2), GGA-untreated UVB-exposed (400 mJ/cm2), GGA-treated (500 mg/kg/mouse) but not exposed and naive controls. Eyeballs were collected 24 h after irradiation, and corneas were stained with hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). HSP70, reactive oxygen species (ROS) production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and protein kinase B (Akt) expression were also evaluated. Irradiated corneal epithelium was significantly thicker in the eyes of mice treated with GGA compared with those given the vehicle alone (p < 0.01). Significantly fewer TUNEL-positive cells were observed in the eyes of GGA-treated mice than controls after irradiation (p < 0.01). Corneal HSP70 levels were significantly elevated in corneas of mice treated with GGA (p < 0.05). ROS signal was not affected by GGA. NF-κB activation was reduced but phospho-(Ser/Ther) Akt substrate expression was increased in corneas after irradiation when treated with GGA. GGA-treatment induced HSP70 expression and ameliorated UV-induced corneal damage through the reduced NF-κB activation and possibly increased Akt phosphorilation.
出版者	en MDPI
日付	Issued2013-01
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/52140
関連	isIdenticalTo DOI https://doi.org/10.3390/ijms14012175
収録誌情報	PISSN 1422-0067 en International Journal of Molecular Sciences 巻14 号1 開始ページ2175 終了ページ2189
ファイル	fulltext IJMS14-1_2175-2189.pdf 3.14 MB (application/pdf) Issued2013-01
コンテンツ更新日時	2023-07-26