A sulfated glycosaminoglycan array for molecular interactions between glycosaminoglycans and growth factors or anti-glycosaminoglycan antibodies

Takada Wataru; Fukushima Masao; Pothacharoen Peraphan; Kongtawelert Prachya; Sugahara Kazuyuki

タイトル	en A sulfated glycosaminoglycan array for molecular interactions between glycosaminoglycans and growth factors or anti-glycosaminoglycan antibodies
作成者	en Takada, Wataru en Fukushima, Masao en Pothacharoen, Peraphan en Kongtawelert, Prachya en Sugahara, Kazuyuki NRID 1000060154449
アクセス権	open access
主題	Other en Antibodies Other en Glycosaminoglycans Other en Growth factors Other en Microarray Other en Molecular interactions NDC 460
内容注記	Abstract en Glycosaminoglycans (GAGS) take part in numerous biological processes by binding to protein molecules and functionally regulating protein-ligand interactions; therefore, molecular interactions of GAGs have been studied by several methods, including surface plasmon resonance, enzyme-linked immunosorbent assays (ELISAs), and GAG microarrays. To achieve rapid, sensitive, and high-throughput screening of GAG interactions, we have developed a novel microarray in which GAGs, including chondroitin sulfate, heparan sulfate, and heparin, were immobilized. The microarray is made from cyclic polyolefin substrate coated with metacrylate polymers, which have phospholipid groups as side chains. The polymer also has aminooxy groups that react specifically with aldehyde groups at the reducing termini of GAG chains, whereas the phospholipid groups prevent nonspecific adsorption of proteins. Thus, minute amounts of GAGs can be chemically immobilized on the surface with low nonspecific binding of proteins. Using this array, interactions between GAGs and antibodies against chondroitin or heparan sulfate and heparin-binding growth factors were examined. The results were in agreement with previously reported specificities, suggesting that the GAG array is useful for high-throughput interaction analyses between GAGs and functional proteins in miniscule amounts and can be applied to both basic studies of GAGs and the development of diagnostic methods for metabolic diseases involving GAGs. (c) 2013 Elsevier Inc. All rights reserved.
出版者	en Elsevier
日付	Issued2013-04-15
言語	eng
資源タイプ	journal article
出版タイプ	AM
資源識別子	HDL http://hdl.handle.net/2115/52650
関連	isVersionOf DOI https://doi.org/10.1016/j.ab.2013.01.004
収録誌情報	PISSN 0003-2697 en Analytical Biochemistry 巻435 号2 開始ページ123 終了ページ130
ファイル	fulltext Supplementary Figure S1.pdf 206.13 KB (application/pdf) Issued2013-04-15 fulltext text Takada20120908 submitted Word.pdf 3.03 MB (application/pdf) Issued2013-04-15
コンテンツ更新日時	2023-07-26