Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.

Yanagida Takashi; Tsushima Jun; Kitamura Yoshihisa; Yanagisawa Daijiro; Takata Kazuyuki; Shibaike Tomonori; Yamamoto Atsuko; Taniguchi Takashi; Yasui Hiroyuki; Taira Takahiro; Morikawa Shigehiro; Inubushi Toshihiro; Tooyama Ikuo; Ariga Hiroyoshi

タイトル	en Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.
作成者	en Yanagida, Takashi en Tsushima, Jun en Kitamura, Yoshihisa NRID 1000060195295 en Yanagisawa, Daijiro en Takata, Kazuyuki NRID 1000010434664 en Shibaike, Tomonori en Yamamoto, Atsuko en Taniguchi, Takashi NRID 1000010111957 en Yasui, Hiroyuki NRID 1000020278443 en Taira, Takahiro NRID 1000070197036 en Morikawa, Shigehiro NRID 1000060220042 en Inubushi, Toshihiro en Tooyama, Ikuo NRID 1000020207533 en Ariga, Hiroyoshi NRID 1000020143505
アクセス権	open access
主題	Other en DJ-1 Other en release Other en astrocytes Other en focal ischemia Other en oxidative stress sensor Other en neuroprotection MeSH en Animals MeSH en Astrocytes/metabolism MeSH en Brain Ischemia/metabolism MeSH en Brain Ischemia/pathology MeSH en Cell Line MeSH en Electron Spin Resonance Spectroscopy MeSH en Humans MeSH en Hydrogen Peroxide/pharmacology MeSH en Hydroxyl Radical/metabolism MeSH en Infarction, Middle Cerebral Artery/metabolism MeSH en Infarction, Middle Cerebral Artery/pathology MeSH en Microtubule-Associated Proteins/genetics MeSH en Microtubule-Associated Proteins/metabolism MeSH en Oxidative Stress MeSH en Rats MeSH en Recombinant Fusion Proteins/pharmacology MeSH en Recombinant Proteins/genetics MeSH en Recombinant Proteins/metabolism NDC 499
内容注記	Abstract en Astrocytes, one of the predominant types of glial cells, function as both supportive and metabolic cells for the brain. Under cerebral ischemia/reperfusion-induced oxidative conditions, astrocytes accumulate and activate in the ischemic region. DJ-1 has recently been shown to be a sensor of oxidative stress in living cells. However, the function of astrocytic DJ-1 is still unknown. In the present study, to clarify the effect of astrocytic DJ-1 protein under massive oxidative insult, we used a focal ischemic rat model that had been subjected to middle cerebral artery occlusion (MCAO) and reperfusion. We then investigated changes in the distribution of DJ-1 in astrocytes, DJ-1 release from cultured astrocytes, and the effects of recombinant DJ-1 protein on hydrogen peroxide (H(2)O(2))-induced death in normal and DJ-1-knockdown SH-SY5Y cells and on in vitro scavenging of hydroxyl radicals (()OH) by electron spin resonance spectrometry. At 24 h after 2-h MCAO and reperfusion, an infarct lesion was markedly observed using magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining. In addition, reactive astrocytes enhanced DJ-1 expression in the penumbral zone of the ischemic core and that DJ-1 protein was extracellularly released from astrocytes by H2O2 in in vitro primary cultures. Although DJ-1-knockdown SH-SY5Y cells were markedly vulnerable to oxidative stress, treatment with glutathione S-transferase-tagged recombinant human DJ-1 protein (GST-DJ-1) significantly inhibited H(2)O(2)-induced cell death. In addition, GST-DJ-1 protein directly scavenged ()OH. These results suggest that oxidative stress induces the release of astrocytic DJ-1 protein, which may contribute to astrocyte-mediated neuroprotection.
出版者	en Hindawi Pub.
日付	Issued2009-01
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/53702
関連	isIdenticalTo DOI https://doi.org/10.4161/oxim.2.1.7985 PMID 20046643
収録誌情報	PISSN 1942-0994 en Oxidative medicine and cellular longevity 巻2 号1 開始ページ36 終了ページ42
ファイル	fulltext 194_Ariga_2009_Oxid_Med_Cell_Long.pdf 2.09 MB (application/pdf) Issued2009-01
コンテンツ更新日時	2023-07-26