Co-localization with DJ-1 is essential for the androgen receptor to exert its transcription activity that has been impaired by androgen antagonists.

Taira Takahiro; Iguchi-Ariga Sanae Marta Margarita; Ariga Hiroyoshi

タイトル	en Co-localization with DJ-1 is essential for the androgen receptor to exert its transcription activity that has been impaired by androgen antagonists.
作成者	en Taira, Takahiro NRID 1000070197036 en Iguchi-Ariga, Sanae Marta Margarita NRID 1000090184283 en Ariga, Hiroyoshi NRID 1000020143505
アクセス権	open access
主題	Other en DJ-1 Other en androgen receptor Other en endocrine disrupter MeSH en Androgen Antagonists/pharmacology MeSH en Androgen Receptor Antagonists MeSH en Animals MeSH en COS Cells MeSH en Cercopithecus aethiops MeSH en Fluorescent Antibody Technique, Indirect MeSH en Humans MeSH en Oncogene Proteins/metabolism MeSH en Oncogene Proteins/physiology MeSH en Receptors, Androgen/metabolism MeSH en Receptors, Androgen/physiology MeSH en Transcription, Genetic NDC 499
内容注記	Abstract en DJ-1 was first identified as a novel candidate of an oncogene product in cooperation with an activated ras, and DJ-1 was later found to be a positive regulator of the androgen receptor (AR) transcription activity that was repressed by PIASxalpha. DJ-1 was also found to be an infertility-related protein that was reduced in rat sperm treated with sperm toxicants that cause infertility in rats. To determine the roles of DJ-1 in the AR function, the effects of several androgen antagonists, some of which had been identified as endocrine-disrupting chemicals, on AR transcription activity and localization of AR and DJ-1 in Cos7 cells were examined. Co-localization of DJ-1 with the AR as dot-like spots in the nucleus was first found in cells that had not been treated with chemicals. Although all of the chemicals tested inhibited AR transcription activity to an average of 25% of that without chemicals, there were two classes affecting the localization of the two proteins; one changes the AR from dot-like spots to diffuse spaces in the nucleus and the other still keeps the AR in the dot-like spots. The localization of DJ-1, on the other hand, was found to be dramatically changed by all of the chemicals, resulting in loss of co-localization with the AR. These results indicate that DJ-1 is an essential factor for the AR to exert its full activity.
出版者	en The Pharmaceutical Society of Japan
日付	Issued2004-04
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/53741
関連	isIdenticalTo DOI https://doi.org/10.1248/bpb.27.574 PMID 15056870
収録誌情報	PISSN 0918-6158 en Biological & pharmaceutical bulletin 巻27 号4 開始ページ574 終了ページ577
ファイル	fulltext 140_Ariga_2004_Biol_Pharm_Bull.pdf 512.0 KB (application/pdf) Issued2004-04
コンテンツ更新日時	2023-07-26