Tissue factor expression in human pterygium.

Ando Ryo; Kase Satoru; Ohashi Tsutomu; Dong Zhenyu; Fukuhara Junichi; Kanda Atsuhiro; Murata Miyuki; Noda Kousuke; Kitaichi Nobuyoshi; Ishida Susumu

タイトル	en Tissue factor expression in human pterygium.
作成者	en Ando, Ryo NRID 1000060399847 en Kase, Satoru NRID 1000060374394 en Ohashi, Tsutomu en Dong, Zhenyu NRID 1000000443945 en Fukuhara, Junichi en Kanda, Atsuhiro NRID 1000080342707 en Murata, Miyuki NRID 1000050423752 en Noda, Kousuke NRID 1000090296666 en Kitaichi, Nobuyoshi NRID 1000040431366 en Ishida, Susumu NRID 1000010245558
アクセス権	open access
主題	MeSH en Actins/biosynthesis MeSH en Conjunctiva/metabolism MeSH en Cytoplasm/metabolism MeSH en Epithelial Cells/metabolism MeSH en Epithelium/pathology MeSH en Formaldehyde/pharmacology MeSH en Gene Expression Regulation MeSH en Humans MeSH en Immunohistochemistry/methods MeSH en Mesoderm/pathology MeSH en Models, Biological MeSH en Pterygium/metabolism MeSH en Receptor, Epidermal Growth Factor/biosynthesis MeSH en Signal Transduction MeSH en Thromboplastin/biosynthesis NDC 496
内容注記	Abstract en Purpose: A pterygium shows tumor-like characteristics, such as proliferation, invasion, and epithelial–mesenchymal transition (EMT). Previous reports suggest that tissue factor (TF) expression is closely related to the EMT of tumor cells, and subsequent tumor development. In this study, we analyzed the expression and immunolocalization of TF in pterygial and normal conjunctival tissues of humans. Methods: Eight pterygia and three normal bulbar conjunctivas, surgically removed, were used in this study. Formalinfixed, paraffin-embedded tissues were submitted for immunohistochemical analysis with anti-TF antibody. Double staining immunohistochemistry was performed to assess TF and alpha-smooth muscle actin (α-SMA) or epidermal growth factor receptor (EGFR) expression in the pterygia. Results: Immunoreactivity for TF was detected in all pterygial tissues examined. TF immunoreactivity was localized in the cytoplasm of basal, suprabasal, and superficial epithelial cells. The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells (p<0.001). TF immunoreactivity was detected in α-SMA-positive or -negative pterygial epithelial cells. EGFR immunoreactivity was detected in pterygial epithelium, which was colocalized with TF. Conclusions: These results suggest that TF plays a potential role in the pathogenesis and development of a pterygium, and that TF expression might be involved through EMT-dependent and -independent pathways.
出版者	en Molecular vision
日付	Issued2011-01-08
言語	eng
資源タイプ	journal article
出版タイプ	VoR
資源識別子	HDL http://hdl.handle.net/2115/54618
関連	PMID 21245964
収録誌情報	PISSN 1090-0535 NCID AA12037116 en Molecular vision 巻17 開始ページ63 終了ページ69
ファイル	fulltext Mol Vis_17_63-69.pdf 3.19 MB (application/pdf) Issued2011-01-08
コンテンツ更新日時	2023-07-26