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Leader cells regulate collective cell migration via Rac activation in the downstream signaling of integrin beta 1 and PI3K
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| 作成者 |
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| アクセス権 |
open access |
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| 内容注記 |
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Abstract
en
Collective cell migration plays a crucial role in several biological processes, such as embryonic development, wound healing, and cancer metastasis. Here, we focused on collectively migrating Madin-Darby Canine Kidney (MDCK) epithelial cells that follow a leader cell on a collagen gel to clarify the mechanism of collective cell migration. First, we removed a leader cell from the migrating collective with a micromanipulator. This then caused disruption of the cohesive migration of cells that followed in movement, called "follower'' cells, which showed the importance of leader cells. Next, we observed localization of active Rac, integrin beta 1, and PI3K. These molecules were clearly localized in the leading edge of leader cells, but not in follower cells. Live cell imaging using active Rac and active PI3K indicators was performed to elucidate the relationship between Rac, integrin beta 1, and PI3K. Finally, we demonstrated that the inhibition of these molecules resulted in the disruption of collective migration. Our findings not only demonstrated the significance of a leader cell in collective cell migration, but also showed that Rac, integrin beta 1, and PI3K are upregulated in leader cells and drive collective cell migration.
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| 出版者 |
en
Nature Publishing Group
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/58058
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.1038/srep07656
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| 収録誌情報 |
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en
Scientific reports
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巻5
開始ページ7656
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
