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タイトル
  • en Two male sterility-inducing cytoplasms of beet (Beta vulgaris) are genetically distinct but have closely related mitochondrial genomes: implication of a substoichiometric mitochondrial DNA molecule in their evolution
作成者
    • en Onodera, Yasuyuki
    • en Arakawa, Takumi
    • en Yui-Kurino, Rika
    • en Yamamoto, Masayuki P
    • en Kitazaki, Kazuyoshi
    • en Ebe, Shigehiko
    • en Matsunaga, Muneyuki
    • en Taguchi, Kazunori
    • en Kuroda, Yosuke
    • en Yamashita, Shiko
    • en Sakai, Tomoyuki
アクセス権 open access
権利情報
  • en The final publication is available at link.springer.com
主題
  • Other en F1 hybrid
  • Other en mitochondrial evolution
  • Other en mitochondrial gene
  • Other en nuclear-cytoplasmic interaction
  • Other en plant mitochondria
  • Other en substoichiometric DNA
  • NDC 467
内容注記
  • Abstract en I-12CMS(2) and I-12CMS(3) are sugar beet lines with different sources of cytoplasmic male sterility (CMS) derived from wild beets in Turkey and Pakistan, respectively. We established that I-12CMS(2) has a genetically distinct cytoplasm, but its mitochondrial genome is very similar to I-12CMS(3). Male fertility was assessed in F1 hybrids produced with a common pollen parent. Fertility in the F1's carrying the I-12CMS(3) cytoplasm exceeded that of the F1's with the I-12CMS(2) cytoplasm. Organization of the I-12CMS(2) and I-12CMS(3) mitochondrial genomes were compared based on their physical maps. Mitochondrial genomes of the two strains were largely collinear, except for a large deletion in the noncoding region of I-12CMS(2). Because a mitochondrial orf129 in the I-12CMS(3) cytoplasm is associated with a male sterility phenotype and preservation of orf129 was evident in I-12CMS(2), I-12CMS(2) orf129 was investigated in detail. I-12CMS(2) plants contained three to five times more ORF129 protein than did I-12CMS(3) plants. A single nucleotide substitution, present in the putative promoter region of orf129, appeared to be responsible for the differential accumulation of orf129 transcript. A long N-terminal extension of atp6 is a common feature of some beet CMSs and is found in I-12CMS(2), but the amino acid sequence is unique. I-12CMS(3) mitochondria, but not I-12CMS(2) mitochondria, were found to be heteroplasmic. This heteroplasmy is characterized by a substoichiometric DNA molecule(s) that has at least two I-12CMS(2)-type mitochondrial loci, suggesting the possibility that the I-12CMS(2) mitochondrial genome might have evolved from such a substoichiometric DNA molecule in I-12CMS(3) mitochondria.
出版者 en Springer
日付
    Issued2015-11
言語
  • eng
資源タイプ journal article
出版タイプ AM
資源識別子 HDL http://hdl.handle.net/2115/63730
関連
  • isVersionOf DOI https://doi.org/10.1007/s10681-015-1484-2
収録誌情報
    • PISSN 0014-2336
      • en Euphytica
      • 巻206 号2 開始ページ365 終了ページ379
ファイル
    • fulltext text1.pdf
    • 1013.59 KB (application/pdf)
      • Issued2015-11
コンテンツ更新日時 2023-07-26