| タイトル |
-
en
Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms
|
| 作成者 |
|
| アクセス権 |
open access |
| 権利情報 |
-
en
This is the peer reviewed version of the following article: Ono, D., Honma, S., Honma, K.-i. (2015), Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms. European Journal of Neuroscience, 42: 3128–3137., which has been published in final form at http://doi.org/10.1111/ejn.13111. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
|
| 主題 |
-
Other
en
behaviour
-
Other
en
bioluminescence
-
Other
en
in vivo recording
-
Other
en
optical fibre
-
Other
en
vasoactive intestinal peptide
-
NDC
490
|
| 内容注記 |
-
Abstract
en
The temporal order of physiology and behaviour in mammals is regulated by the coordination of the master circadian clock in the suprachiasmatic nucleus (SCN) and peripheral clocks in various tissues outside the SCN. Because the circadian oscillator(s) in the olfactory bulb (OB) is regarded as SCN independent, we examined the relationship between the SCN master clock and the circadian clock in the OB. We also examined the role of vasoactive intestinal peptide receptor 2 in the circadian organization of the OB. We continuously monitored the circadian rhythms of a clock gene product PER2 in the SCN and OB of freely moving mice by means of a bioluminescence reporter and an optical fibre implanted in the brain. Robust circadian rhythms were detected in the OB and SCN for up to 19 days. Bilateral SCN lesions abolished the circadian behaviour rhythms and disorganized the PER2 rhythms in the OB. The PER2 rhythms in the OB showed more than one oscillatory component of a similar circadian period, suggesting internal desynchronization of constituent oscillators. By contrast, significant circadian PER2 rhythms were detected in the vasoactive intestinal peptide receptor 2-deficient mice, despite the substantial deterioration or abolition of circadian behavioural rhythms. These findings indicate that the circadian clock in the OB of freely moving mice depends on the SCN master clock but not on the circadian behavioural rhythms. The circadian PER2::LUC rhythm in the cultured OB was as robust as that in the cultured SCN but reset by slice preparation, suggesting that culturing of the slice reinforces the circadian rhythm.
|
| 出版者 |
en
Wiley-Blackwell
|
| 日付 |
|
| 言語 |
|
| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/63844
|
| 関連 |
-
isVersionOf
DOI
https://doi.org/10.1111/ejn.13111
-
PMID
26489367
|
| 収録誌情報 |
-
-
en
European journal of neuroscience
-
巻42
号12
開始ページ3128
終了ページ3137
|
| ファイル |
|
| コンテンツ更新日時 |
2023-07-26 |
