| タイトル |
-
Overexpression of Galnt3 in Chondrocytes Resulted in Dwarfism Due to the Increase of Mucin-type O-Glycans and Reduction of Glycosaminoglycans
|
|---|
| 作成者 |
|
|---|
| 権利情報 |
-
-
This research was originally published in Journal of Biological Chemistry. Carolina Andrea Yoshida, Tetsuya Kawane, Takeshi Moriishi, Anurag Purushothaman, Toshihiro Miyazaki, Hisato Komori, Masako Mori, Xin Qin, Ayako Hashimoto, Kazuyuki Sugahara, Kei Yamana, Kenji Takada and Toshihisa Komori. Overexpression of Galnt3 in Chondrocytes Resulted in Dwarfism Due to the Increase of Mucin-type O-Glycans and Reduction of Glycosaminoglycans. Journal of Biological Chemistry. 2014; Vol:289(38) p26584-26596. © the American Society for Biochemistry and Molecular Biology
|
|---|
| 主題 |
-
Other
Glycosyltransferase
-
Other
Mucin-type O-Glycan
|
|---|
| 内容注記 |
Other
-
Galnt3, UDP-N-acetyl-α-d-polypeptide N-acetylgalactosaminyltransferase 3, transfers N-acetyl-d-galactosamine to serine and threonine residues, initiating mucin type O-glycosylation of proteins. We searched the target genes of Runx2, which is an essential transcription factor for chondrocyte maturation, in chondrocytes and found that Galnt3 expression was up-regulated by Runx2 and severely reduced in Runx2−/− cartilaginous skeletons. To investigate the function of Galnt3 in chondrocytes, we generated Galnt3−/− mice and chondrocyte-specific Galnt3 transgenic mice under the control of the Col2a1 promoter-enhancer. Galnt3−/− mice showed a delay in endochondral ossification and shortened limbs at embryonic day 16.5, suggesting that Galnt3 is involved in chondrocyte maturation. Galnt3 transgenic mice presented dwarfism, the chondrocyte maturation was retarded, the cell cycle in chondrocytes was accelerated, premature chondrocyte apoptosis occurred, and the growth plates were disorganized. The binding of Vicia villosa agglutinin, which recognizes the Tn antigen (GalNAc-O-Ser/Thr), was drastically increased in chondrocytes, and aggrecan (Acan) was highly enriched with Tn antigen. However, safranin O staining, which recognizes glycosaminoglycans (GAGs), and Acan were severely reduced. Chondroitin sulfate was reduced in amount, but the elongation of chondroitin sulfate chains had not been severely disturbed in the isolated GAGs. These findings indicate that overexpression of Galnt3 in chondrocytes caused dwarfism due to the increase of mucin-type O-glycans and the reduction of GAGs, probably through competition with xylosyltransferases, which initiate GAG chains by attaching O-linked xylose to serine residues, suggesting a negative effect of Galnt family proteins on Acan deposition in addition to the positive effect of Galnt3 on chondrocyte maturation.
|
|---|
| 出版者 | American Society for Biochemistry and Molecular Biology (ASBMB)
|
|---|
| 日付 | Issued 2014-09-19
|
|---|
| 言語 | eng |
|---|
| 資源タイプ | journal article |
|---|
| 出版タイプ | VoR |
|---|
| 資源識別子 | URI http://hdl.handle.net/2115/62911
|
|---|
| 関連 |
-
isIdenticalTo
PMID
25107907
-
isIdenticalTo
DOI
https://doi.org/10.1074/jbc.M114.555987
|
|---|
| 収録誌情報 | -
-
Journal of Biological Chemistry
289(38), 26584-26596
|
|---|
| ファイル |
|
|---|
| コンテンツ更新日時 | 2019-10-09T00:14:31Z |
|---|
