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タイトル
  • en Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines
作成者
    • en Muramatsu, Daisuke
    • en Kawata, Koji
    • en Aoki, Shiho
    • en Uchiyama, Hirofumi
    • en Okabe, Mitsuyasu
    • en Iwai, Atsushi
アクセス権 open access
権利情報
主題
  • Other en Extracellular signalling molecules
  • Other en Interferons
  • NDC 491
内容注記
  • Abstract en A β-(1,3),(1,6)-D-glucan produced by A. pullulans (AP-PG) is known to be an immune stimulating agent. In this study, we demonstrate that the stimulation with AP-PG effectively induces the interferon (IFN) stimulated genes (ISGs) in macrophage-like cell lines. The ISGs, Mx1, ISG15, and viperin mRNAs were significantly increased in RAW264.7 cells after stimulation with AP-PG. The stimulation with AP-PG transiently induced IFN-β mRNA. However, the expression of viperin mRNA was also increased after stimulation with AP-PG even when new protein synthesis was completely blocked by treatment with cycloheximide. Further, in IFN-α receptor knockdown RAW264.7 cells, AP-PG stimulation more effectively induced viperin mRNA compared with that of IFN-α stimulation. The phosphorylation of Ser 727 in STAT1 involved in the enhancement of STAT1 activation was immediately increased after stimulation with AP-PG. In addition, viperin mRNA expression induced after stimulation with IFN-α was significantly increased by combined stimulation with AP-PG. These results suggest that stimulation with AP-PG effectively induces the ISGs through the induction of IFN and the enhancement of STAT1-mediated transcriptional activation.
出版者 en Nature
日付
    Issued2014-04-24
言語
  • eng
資源タイプ journal article
出版タイプ VoR
資源識別子 HDL http://hdl.handle.net/2115/63432
関連
  • isIdenticalTo DOI https://doi.org/10.1038/srep04777
収録誌情報
    • EISSN 2045-2322
      • en Scientific Reports
      • 巻4 開始ページ4777
ファイル
コンテンツ更新日時 2023-07-26