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タイトル
  • en Two types of amorphous protein particles facilitate crystal nucleation
作成者
    • en Yamazaki, Tomoya
    • en Vekilov, Peter G.
    • en Furukawa, Erika
    • en Shirai, Manabu
    • en Matsumoto, Hiroaki
    • en Van Driessche, Alexander E. S.
    • en Tsukamoto, Katsuo
アクセス権 open access
主題
  • Other en nucleation
  • Other en protein
  • Other en lysozyme
  • Other en transmission electron microscopy
  • Other en in situ observation
内容注記
  • Abstract en Nucleation, the primary step in crystallization, dictates the number of crystals, the distribution of their sizes, the polymorph selection, and other crucial properties of the crystal population. We used timeresolved liquid-cell transmission electron microscopy (TEM) to perform an in situ examination of the nucleation of lysozyme crystals. Our TEM images revealed that mesoscopic clusters, which are similar to those previously assumed to consist of a dense liquid and serve as nucleation precursors, are actually amorphous solid particles (ASPs) and act only as heterogeneous nucleation sites. Crystalline phases never form inside them. We demonstrate that a crystal appears within a noncrystalline particle assembling lysozyme on an ASP or a container wall, highlighting the role of heterogeneous nucleation. These findings represent a significant departure from the existing formulation of the two-step nucleation mechanism while reaffirming the role of noncrystalline particles. The insights gained may have significant implications in areas that rely on the production of protein crystals, such as structural biology, pharmacy, and biophysics, and for the fundamental understanding of crystallization mechanisms.
出版者 en National Academy of Sciences.
日付
    Issued2017-01-10
言語
  • eng
資源タイプ journal article
出版タイプ AM
資源識別子 HDL http://hdl.handle.net/2115/66517
関連
  • isVersionOf DOI https://doi.org/10.1073/pnas.1606948114
収録誌情報
    • PISSN 0027-8424
      • en Proceedings of the National Academy of Sciences of the United States of America (PNAS)
      • 114 9 開始ページ2154 終了ページ2159
ファイル
コンテンツ更新日時 2023-07-26