| タイトル |
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en
Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts
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| 作成者 |
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| アクセス権 |
open access |
| 権利情報 |
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| 主題 |
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| 内容注記 |
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Abstract
en
Stress fibers (SFs) are contractile, force-generating bundled structures that can be classified into three subtypes, namely ventral SFs (vSFs), transverse arcs (TAs), and dorsal SFs. Nonmuscle myosin II (NMII) is the main component of SFs. This study examined the roles of the NMII isoforms NMIIA and NMIIB in the organization of each SF subtype in immortalized fibroblasts. Knockdown (KD) of NMIIA (a major isoform) resulted in loss of TAs from the lamella and caused the lamella to lose its flattened shape. Exogenous expression of NMIIB rescued this defect in TA formation. However, the TAs that formed on exogenous NMIIB expression in NMIIA-KD cells and the remaining TAs in NMIIB-KD cells, which mainly consisted of NMIIB and NMIIA, respectively, failed to rescue the defect in lamellar flattening. These results indicate that both isoforms are required for the proper function of TAs in lamellar flattening. KD of NMIIB resulted in loss of vSFs from the central region of the cell body, and this defect was not rescued by exogenous expression of NMIIA, indicating that NMIIA cannot replace the function of NMIIB in vSF formation. Moreover, we raised the possibility that actin filaments in vSFs are in a stretched conformation.
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| 出版者 |
en
American Society for Cell Biology
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
VoR |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/71475
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| 関連 |
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isIdenticalTo
DOI
https://doi.org/10.1091/mbc.E17-04-0215
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| 収録誌情報 |
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en
Molecular biology of the cell
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巻29
号8
開始ページ911
終了ページ922
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
