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タイトル	en A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells
作成者	en Fujioka, Yoichiro en Nishide, Shinya NRID 1000040451398 en Ose, Toyoyuki NRID 1000080380525 en Suzuki, Tadaki NRID 1000030527180 en Kato, Izumi en Fukuhara, Hideo en Fujioka, Mari en Horiuchi, Kosui en Satoh, Aya O. en Nepal, Prabha en Kashiwagi, Sayaka en Wang, Jing en Horiguchi, Mika en Sato, Yuko en Paudel, Sarad en Nanbo, Asuka NRID 1000060359487 en Miyazaki, Tadaaki NRID 1000060272431 en Hasegawa, Hideki en Maenaka, Katsumi NRID 1000010322752 en Ohba, Yusuke NRID 1000030333503
アクセス権	open access
権利情報	en © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ en Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
主題	Other en influenza A virus Other en virus entry Other en calcium ion Other en calcium channel Other en calcium channel blockers Other en hemagglutinin Other en sialylation Other en virus-host cell interaction NDC 490
内容注記	Abstract en Influenza A virus (IAV) infection is initiated by the attachment of the viral glycoprotein hemagglutinin (HA) to sialic acid on the host cell surface. However, the sialic acid-containing receptor crucial for IAV infection has remained unidentified. Here, we show that HA binds to the voltage-dependent Ca2+ channel Cav1.2 to trigger intracellular Ca2+ oscillations and subsequent IAV entry and replication. IAV entry was inhibited by Ca2+ channel blockers (CCBs) or by knockdown of Cav1.2. The CCB diltiazem also inhibited virus replication in vivo. Reintroduction of wild-type but not the glycosylation-deficient mutants of Cav1.2 restored Ca2+ oscillations and virus infection in Cav1.2-depleted cells, demonstrating the significance of Cav1.2 sialylation. Taken together, we identify Cav1.2 as a sialylated host cell surface receptor that binds HA and is critical for IAV entry.
出版者	en Cell Press
日付	Issued2018-06-13
言語	eng
資源タイプ	journal article
出版タイプ	AM
資源識別子	HDL http://hdl.handle.net/2115/74619
関連	isVersionOf DOI https://doi.org/10.1016/j.chom.2018.04.015
収録誌情報	PISSN 1931-3128 en Cell host & microbe 巻23 号6 開始ページ809 終了ページ818
ファイル	fulltext 03_CHM-17-00837_Suppl.pdf 7.9 MB (application/pdf) Issued2018-06-13 fulltext 04_CHM-17-00837_Table_S1.docx 16.39 KB (application/vnd.openxmlformats-officedocument.wordprocessingml.document) Issued2018-06-13 fulltext 1-s2.0-S1931312818302166-mmc2.mp4 2.89 MB (video/mp4) Issued2018-06-13 fulltext 1-s2.0-S1931312818302166-mmc3.mp4 2.9 MB (video/mp4) Issued2018-06-13 fulltext CellHostMicrobe23_809.pdf 1.32 MB (application/pdf) Issued2018-06-13
コンテンツ更新日時	2023-07-26