| タイトル |
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en
Simple enumeration of Escherichia coli concentrations in river water samples by measuring beta-d-glucuronidase activities in a microplate reader
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| 作成者 |
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| アクセス権 |
open access |
| 主題 |
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Other
en
enzyme
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Other
en
fecal pollution
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Other
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fluctuation
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Other
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fluorescence
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Other
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Sapporo
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Other
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wastewater
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NDC
519
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| 内容注記 |
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Abstract
en
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the beta-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL(-1) with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW.
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| 出版者 |
en
IWA Publishing
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| 日付 |
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| 言語 |
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| 資源タイプ |
journal article |
| 出版タイプ |
AM |
| 資源識別子 |
HDL
http://hdl.handle.net/2115/81524
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| 関連 |
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isVersionOf
DOI
https://doi.org/10.2166/wst.2021.072
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| 収録誌情報 |
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en
Water Science and Technology
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巻83
号6
開始ページ1399
終了ページ1406
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| ファイル |
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| コンテンツ更新日時 |
2023-07-26 |
