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Title
  • ja Comparison of Two Different Methods for the Purification of Polymerase Chain Reaction (PCR) Products Used in Direct Sequencing, in an Applied Biosystems 373A DNA Sequencing System
Alternative
  • Institute of Tropical Medicine, Nagasaki University
Creator
    • Thayan, Ravindran
    • Morita, Kouichi
    • Vijayamalar, Balasubramaniam
    • Chew, Tong Keong
    • Sinniah, Mangalam
    • Igarashi, Akira
Description
  • Other This study was carried out to compare the efficiency of two different purification methods used for removing excess primers and short oligonucleotides from PCR products. Our aim was to sequence PCR product directly. The two different purification methods were micro filter spin unit system (Millipore) and Quick Spin Column (Boehringer Mannheim Biochemica). The results indicated that the purification method using microfilter spin unit system was better and yielded better suquencing pattern compared to quick Spin Column.
  • Other identifier:熱帯医学 Tropical medicine 37(1). p29-33, 1995
Publisher 長崎大学熱帯医学研究所
Date
    Created2020-12-24 , Issued1995-06-23
Language
  • eng
Resource Type departmental bulletin paper
Version Type NA
Identifier URI http://hdl.handle.net/10069/4701
Journal
    • NCID AN00199644
    • ISSN 0385-5643
      • 熱帯医学 Tropical medicine 37(1), 29-33
File
Oaidate 2021-02-17