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Title
  • Immunohistochemical analysis of connexin43 expression in infertile human testes.
Creator

Matsuo, Yuzo

Nomata, Koichiro

Eguchi, Jiro

Aoki, Daiyu

Hayashi, Tomayoshi

Hishikawa, Yoshitaka

Kanetake, Hiroshi

Shibata, Yoshisada

Koji, Takehiko

Rights
    • Copyright ©2007 By the Japan Society of Histochemistry and Cytochemistry
Subject
  • Other connexin43
  • Other Johnsen score
  • Other infertile human testes
Description
Other
  • Connexin43 (Cx43) is abundantly expressed in mammalian testes and implicated in the regulation of cell-to-cell interaction between germ cells and Sertoli cells, which is essential to the normal process of spermatogenesis. In the present study, we investigated the relation between Cx43 expression and the degree of spermatogenesis in infertile human testes. Immunohistochemical analysis of Cx43 was performed on testicular biopsies from 29 patients with azoospermia (n=23) and severe oligospermia (n=6), who gave informed consent to this experiment. The degree of testicular spermatogenesis was evaluated by Johnsen score. In the interstitium, immunostaining for Cx43 was localized to some focal parts of plasma membrane between neighboring Leydig cells. In seminiferous tubules with normal spermatogenesis, Cx43 expression was found between Sertoli cells and germ cells. However, Cx43 expression in maturation arrest was decreased and located mainly in the basal compartment of seminiferous tubules. Finally, there was a significant positive correlation between histological score of spermatogenesis and intensity of Cx43 (p=0.0294). These data suggest that the alteration of Cx43 expression may be involved in spermatogenic impairment, and that the communication between Sertoli cells and germ cells through Cx43 may be important for maturation of spermatogenesis.
Other
  • identifier:Acta Histochemica et Cytochemica, 40(3), pp.69-75; 2007
Other
  • identifier:13475800
PublisherThe Japan Society of Histochemistry and Cytochemistry
Date Issued 2007-07-03
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/10069/23716
Relation
  • isIdenticalTo PMID 17653298
  • isIdenticalTo DOI https://doi.org/10.1267/ahc.07001
Journal
    • ISSN 0044-5991
    • Acta histochemica et cytochemica
    40(3), 69-75
File
Oaidate2012-01-26T06:48:07Z