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Title
  • Serum Starvation Activates NF-κB Through G Protein β2 Subunit-Mediated Signal
Creator

Kohno, Tomoko

Kubo, Yoshinao

Yasui, Kiyoshi

Haraguchi, Megumi

Shigematsu, Sayuri

Chua, Koon Jiew

Matsuyama, Toshifumi

Hayashi, Hideki

Rights
    • This is a copy of an article published in the DNA and Cell Biology © 2012 Mary Ann Liebert, Inc.; DNA and Cell Biology is available online at: http://online.liebertpub.com.
Description
Other
  • Several cell stresses induce nuclear factor-kappaB (NF-κB) activation, which include irradiation, oxidation, and UV. Interestingly, serum-starving stress-induced NF-κB activation in COS cells, but not in COS-A717 cells. COSA717 is a mutant cell line of COS cells that is defective of the NF-κB signaling pathway. We isolated genes with compensating activity for the NF-κB pathway and one gene encoded the G protein b2 (Gb2). Gb2 is one of the G rotein-coupled receptor signaling effectors. In COS-A717 cells, Gb2 expression is significantly reduced. In Gb2 cDNA-transfected COS-A717 cells, the NF-κB activity was increased along with the recovery of Gb2 expression. Furthermore, serum-starving stress induced the NF-κB activity in Gb2-transfected COS-A717 cells. Consistently, the serum-starved COS cells with siRNA-reduced Gb2 protein expression showed decreased NF-κB activity. These results indicate that Gb2 is required for starvation-induced NF-κB activation and constitutive NF-kB activity. We propose that serum contains some molecule(s) that strongly inhibits NF-κB activation mediated through Gβ2 signaling.
Other
  • identifier:DNA and Cell Biology, 31(11), pp.1636-1644; 2012
Other
  • identifier:15577430
PublisherMary Ann Liebert Inc.
Date Issued 2012-11-01
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/10069/32434
Relation
  • isIdenticalTo DOI https://doi.org/10.1089/dna.2012.1666
Journal
    • ISSN 1044-5498
    • DNA and Cell Biology
    31(11), 1636-1644
File
Oaidate2013-06-24T14:15:50Z