• Susceptibility of muridae cell lines to ecotropic murine leukemia virus and the cationic amino acid transporter 1 viral receptor sequences: implications for evolution of the viral receptor

Kakoki, Katsura

Shinohara, Akio

Izumida, Mai

Koizumi, Yosuke

Honda, Eri

Kato, Goro

Igawa, Tsukasa

Sakai, Hideki

Hayashi, Hideki

Matsuyama, Toshifumi

Morita, Tetsuo

Koshimoto, Chihiro

Kubo, Yoshinao

    • © Springer Science+Business Media 2014.
    • The final publication is available at
  • Other CAT1
  • Other Ecotropic murine leukemia virus
  • Other Evolution
  • Other Glycosylation
  • Ecotropic murine leukemia viruses (Eco-MLVs) infect mouse and rat, but not other mammalian cells, and gain access for infection through binding the cationic amino acid transporter 1 (CAT1). Glycosylation of the rat and hamster CAT1s inhibits Eco-MLV infection, and treatment of rat and hamster cells with a glycosylation inhibitor, tunicamycin, enhances Eco-MLV infection. Although the mouse CAT1 is also glycosylated, it does not inhibit Eco-MLV infection. Comparison of amino acid sequences between the rat and mouse CAT1s shows amino acid insertions in the rat protein near the Eco-MLV-binding motif. In addition to the insertion present in the rat CAT1, the hamster CAT1 has additional amino acid insertions. In contrast, tunicamycin treatment of mink and human cells does not elevate the infection, because their CAT1s do not have the Eco-MLV-binding motif. To define the evolutionary pathway of the Eco-MLV receptor, we analyzed CAT1 sequences and susceptibility to Eco-MLV infection of other several murinae animals, including the southern vole (Microtus rossiaemeridionalis), large Japanese field mouse (Apodemus speciosus), and Eurasian harvest mouse ( Micromys minutus). Eco-MLV infection was enhanced by tunicamycin in these cells, and their CAT1 sequences have the insertions like the hamster CAT1. Phylogenetic analysis of mammalian CAT1s suggested that the ancestral CAT1 does not have the Eco-MLV-binding motif, like the human CAT1, and the mouse CAT1 is thought to be generated by the amino acid deletions in the third extracellular loop of CAT1.
  • identifier:Virus Genes, 48(3), pp.448-456; 2014
  • identifier:1572994X
PublisherSpringer Netherlands
Date Issued 2014-06
NIItypejournal article
Identifier URI
  • isIdenticalTo DOI
    • ISSN 0920-8569
    • Virus Genes
    48(3), 448-456