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Title
  • Rab3a-Bound CD63 Is Degraded and Rab3a-Free CD63 Is Incorporated into HIV-1 Particles
Creator

Kubo, Yoshinao

Masumoto, Hiroshi

Izumida, Mai

Kakoki, Katsura

Hayashi, Hideki

Matsuyama, Toshifumi

Rights
    • © 2017 Kubo, Masumoto, Izumida, Kakoki, Hayashi and Matsuyama. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Subject
  • Other CD63
  • Other HIV-1
  • Other Rab3a
  • Other virion formation
  • Other endosome/lysosome
Description
Other
  • CD63, a member of the tetraspanin family, is involved in virion production by human immunodeficiency virus type 1 (HIV-1), but its mechanism is unknown. In this study, we showed that a small GTP-binding protein, Rab3a, interacts with CD63. When Rab3a was exogenously expressed, the amounts of CD63 decreased in cells. The Rab3a-mediated reduction of CD63 was suppressed by lysosomal and proteasomal inhibitors. The amount of CD63 was increased by reducing the endogenous Rab3a level using a specific shRNA. These results indicate that Rab3a binds to CD63 to induce the degradation of CD63. Rab3a is thought to be involved in exocytosis, but we found that another function of Rab3a affects the fate of CD63 in lysosomes. CD63 interacted with Rab3a and was incorporated into HIV-1 particles. However, Rab3a was not detected in HIV-1 virions, thereby indicating that Rab3a-free CD63, but not Rab3a-bound CD63, is incorporated into HIV-1 particles. Overexpression or silencing of Rab3a moderately reduced HIV-1 virion formation. Overexpression of Rab3a decreased CD63 levels, but did not affect the incorporation of CD63 into HIV-1 particles. This study showed that Rab3a binds to CD63 to induce the degradation of CD63, and only Rab3a-free CD63 is incorporated into HIV-1 particles.
Other
  • identifier:Frontiers in Microbiology, 8, 1653; 2017
Other
  • identifier:1664302X
PublisherFrontiers Media S. A.
Date Issued 2017-08-29
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/10069/37777
Relation
  • isIdenticalTo DOI https://doi.org/10.3389/fmicb.2017.01653
Journal
    • Frontiers in Microbiology
    8, 1653
File
Oaidate2017-09-25T14:03:04Z