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Title
  • Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis
Creator

Izumida, Mai

Hayashi, Hideki

Tanaka, Atsushi

Kubo, Yoshinao

Rights
    • © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Subject
  • Other cathepsin B
  • Other chikungunya viruse
  • Other endocytosis
  • Other macropinocytosis
  • Other murine leukemia virus vector
Description
Other
  • Chikungunya virus (CHIKV) is an enveloped virus that enters host cells and transits
    within the endosomes before starting its replication cycle, the precise mechanism of which is yet to be elucidated. Endocytosis and endosome acidification inhibitors inhibit infection by CHIKV,murine leukemia virus (MLV), or SARS-coronavirus, indicating that these viral entries into host cells
    occur through endosomes and require endosome acidification. Although endosomal cathepsin B
    protease is necessary for MLV, Ebola virus, and SARS-CoV infections, its role in CHIKV infection is unknown. Our results revealed that endocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in 293T cells but not in TE671 cells. In contrast, macropinocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in TE671 cells but not in 293T cells, suggesting
    that CHIKV host cell entry occurs via endocytosis or macropinocytosis, depending on the cell lines used. Cathepsin B inhibitor and knockdown by an shRNA suppressed CHIKV-pseudotyped MLV vector infection both in 293T and TE671 cells. These results show that cathepsin B facilitates CHIKV infection regardless of the entry pathway.
Other
  • identifier:Viruses, 12(7), art.no.722; 2020
Other
  • identifier:19994915
PublisherMDPI
Date Issued 2020-07-03
Languageeng
NIItypejournal article
VersiontypeVoR
Identifier URI http://hdl.handle.net/10069/40150
Relation
  • isIdenticalTo DOI https://doi.org/10.3390/v12070722
Journal
    • Viruses
    12(7), 722
File
Oaidate2020-07-22T14:03:32Z