• ja Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis
    • Izumida, Mai
    • Hayashi, Hideki
    • Tanaka, Atsushi
    • Kubo, Yoshinao
  • c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
  • Other cathepsin B
  • Other chikungunya viruse
  • Other endocytosis
  • Other macropinocytosis
  • Other murine leukemia virus vector
  • Other Chikungunya virus (CHIKV) is an enveloped virus that enters host cells and transitswithin the endosomes before starting its replication cycle, the precise mechanism of which is yet to be elucidated. Endocytosis and endosome acidification inhibitors inhibit infection by CHIKV,murine leukemia virus (MLV), or SARS-coronavirus, indicating that these viral entries into host cellsoccur through endosomes and require endosome acidification. Although endosomal cathepsin Bprotease is necessary for MLV, Ebola virus, and SARS-CoV infections, its role in CHIKV infection is unknown. Our results revealed that endocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in 293T cells but not in TE671 cells. In contrast, macropinocytosis inhibitors attenuated CHIKV-pseudotyped MLV vector infection in TE671 cells but not in 293T cells, suggestingthat CHIKV host cell entry occurs via endocytosis or macropinocytosis, depending on the cell lines used. Cathepsin B inhibitor and knockdown by an shRNA suppressed CHIKV-pseudotyped MLV vector infection both in 293T and TE671 cells. These results show that cathepsin B facilitates CHIKV infection regardless of the entry pathway.
  • Other identifier:Viruses, 12(7),; 2020
Publisher MDPI
    Created2020-12-18 , Issued2020-07-03
  • eng
Resource Type journal article
Version Type VoR
Identifier URI
  • isIdenticalTo DOI
    • ISSN 1999-4915
      • Viruses 12(7), 722
Oaidate 2021-02-17