Title |
-
en
Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
|
Creator |
|
Accessrights |
open access |
Rights |
-
en
The original publication is available at www.springerlink.com
|
Subject |
-
Other
en
Chondrocyte
-
Other
en
Differentiation
-
Other
en
Mesenchymal stem cell
-
Other
en
Three-dimension
-
NDC
481.1
|
Description |
-
Abstract
en
A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-l-lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture with growth factors, namely, dexamethasone and transforming growth factor (TGF)-β 3. The 3D culture for 2 week with the combined addition of dexamethasone, TGF-β 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than 2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week.
|
Publisher |
en
Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
|
Date |
|
Language |
|
Resource Type |
journal article |
Version Type |
AM |
Identifier |
HDL
http://hdl.handle.net/2115/922
|
Relation |
-
isVersionOf
DOI
https://doi.org/10.1007/s10616-005-3751-x
|
Journal |
-
-
PISSN
0920-9069
-
EISSN
1573-0778
-
en
Cytotechnology
-
Volume Number47
Issue Number1-3
Page Start11
Page End17
|
File |
|
Oaidate |
2023-07-26 |