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Title
  • en Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
Creator
    • en Matsuda, Chikayoshi
    • en Hattori, Takako
    • en Wakitani, Shigeyuki
    • en Yoshida, Toshiomi
Accessrights open access
Rights
  • en The original publication is available at www.springerlink.com
Subject
  • Other en Chondrocyte
  • Other en Differentiation
  • Other en Mesenchymal stem cell
  • Other en Three-dimension
  • NDC 481.1
Description
  • Abstract en A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-l-lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture with growth factors, namely, dexamethasone and transforming growth factor (TGF)-β 3. The 3D culture for 2 week with the combined addition of dexamethasone, TGF-β 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than 2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with the 3D cultivation for 2 week.
Publisher en Springer Science+Business Media B.V., Formerly Kluwer Academic Publishers B.V.
Date
    Issued2005-01
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/922
Relation
  • isVersionOf DOI https://doi.org/10.1007/s10616-005-3751-x
Journal
    • PISSN 0920-9069
    • EISSN 1573-0778
      • en Cytotechnology
      • Volume Number47 Issue Number1-3 Page Start11 Page End17
File
    • fulltext CT47(1-3).pdf
    • 110.0 KB (application/pdf)
      • Issued2005-01
Oaidate 2023-07-26