A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR
A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0～2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0～8.0> times higher than those by virus isolation or by RT-PCR.
|Publisher||The Graduate School of Veterinary Medicine
|Date|| Issued 2001-02-28
|NIItype||departmental bulletin paper|
|Identifier|| URI http://hdl.handle.net/2115/2859
, DOI https://doi.org/10.14943/jjvr.48.4.187
Japanese Journal of Veterinary Research