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Title
  • A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR
Creator

OZAKI, Hiroichi

SUGITA, Shigeo

KIDA, Hiroshi

Subject
  • NDC 649
Description
Other
  • A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0~2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0~8.0> times higher than those by virus isolation or by RT-PCR.
PublisherThe Graduate School of Veterinary Medicine
Date Issued 2001-02-28
Languageeng
NIItypedepartmental bulletin paper
VersiontypeVoR
Identifier URI http://hdl.handle.net/2115/2859 , DOI https://doi.org/10.14943/jjvr.48.4.187
ID

JaLC10.14943/jjvr.48.4.187

Journal
    • NCID AA0069128X
    • ISSN 0047-1917
    • Japanese Journal of Veterinary Research
    48(4), 187-195
File
Oaidate2019-04-25T02:49:56Z