CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype

Baba Tomohisa; Ishizu Akihiro; 石津 明洋; Iwasaki Sari; Suzuki Akira; Tomaru Utano; Ikeda Hitoshi; Yoshiki Takashi; Kasahara Masanori

Title	en CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype
Creator	en Baba, Tomohisa en Ishizu, Akihiro ja 石津, 明洋 NRID 1000060321957 en Iwasaki, Sari NRID 1000060455631 en Suzuki, Akira en Tomaru, Utano NRID 1000020360901 en Ikeda, Hitoshi en Yoshiki, Takashi NRID 1000060220612 en Kasahara, Masanori NRID 1000030241318
Accessrights	open access
Subject	NDC 493.1
Description	Abstract en We found a population of nonlymphoid cells expressing both CD4 and CD8 in peripheral blood mononuclear cells (PBMCs) of human T-cell leukemia virus type-I pX transgenic rats with autoimmune diseases. These cells, which showed a monocytic phenotype, were also found in wild-type rats, and their number increased by adjuvant-assisted immunization. GM-CSF increased the number of these double-positive (DP) monocytes in PBMCs. Consistent with the idea that DP monocytes differentiate into DP macrophages at sites of inflammation, we found infiltration of DP macrophages at the site of myosin-induced myocarditis in wild-type rats; these cells exhibited a T-helper 1 (Th1)-type cytokine/chemokine profile and expressed high levels of Fas ligand, perforin, granzyme B, and NKR-P2 (rat orthologue of human NKG2D). Adoptive transfer of GFP-positive spleen cells confirmed hematogenous origin of DP macrophages. DP monocytes had a cytotoxic phenotype similar to DP macrophages, indicating that this phenotypic specialization occurred before entry into a tissue. In line with this, DP monocytes killed tumor cells in vitro. Combined evidence indicates that certain inflammatory stimuli that induce GM-CSF trigger the expansion of a population of DP monocytes with a cytotoxic phenotype and that these cells differentiate into macrophages at inflammatory sites. Interestingly, human PBMCs also contain DP monocytes. Other http://www.bloodjournal.org/
Publisher	en American Society of Hematology.
Date	Issued2006
Language	eng
Resource Type	journal article
Version Type	AM
Identifier	HDL http://hdl.handle.net/2115/5924
Relation	isVersionOf DOI https://doi.org/10.1182/blood-2005-06-2345 PMID 16269616
Journal	PISSN 0006-4971 en Blood Volume Number107 Issue Number5 Page Start2004 Page End2012
File	fulltext B107-5.pdf 1.61 MB (application/pdf) Issued2006
Oaidate	2023-07-26