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Title
  • en Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections.
Creator
Accessrights open access
Rights
  • en The original publication is available at www.springerlink.com
Subject
  • Other en Acellular cementum
  • Other en Fresh-frozen sections
  • Other en Matrix vesicles
  • Other en Mineralization
  • Other en Tissue non-specific alkaline phosphatase
  • NDC 497
Description
  • Abstract en This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis (2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP- and OPN-immunoreactive, initial cementum layer appeared. At this point, cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: during the initial cementogenesis, cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment, mineralization advances along collagen fibrils without matrix vesicles.
Publisher en Springer
Date
    Issued2007-03
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/17088
Relation
  • isVersionOf DOI https://doi.org/10.1007/s00418-006-0242-x
  • PMID 17043865
Journal
    • PISSN 0948-6143
    • EISSN 1432-119X
      • en Histochemistry and Cell Biology
      • Volume Number127 Issue Number3 Page Start303 Page End311
File
    • fulltext H&CB.pdf
    • 639.29 KB (application/pdf)
      • Issued2007-03
Oaidate 2023-07-26