Differences in sialic acid-galactose linkages in the chicken egg amnion and allantois influence human influenza virus receptor specificity and variant selection

Ito T.; Suzuki Y.; Takada A.; Kawamoto A.; Otsuki K.; Masuda H.; Yamada M.; Suzuki T.; Kida H.; Kawaoka Y.

Title	en Differences in sialic acid-galactose linkages in the chicken egg amnion and allantois influence human influenza virus receptor specificity and variant selection
Creator	en Ito, T. en Suzuki, Y. en Takada, A. NRID 1000010292062 en Kawamoto, A. en Otsuki, K. en Masuda, H. en Yamada, M. en Suzuki, T. en Kida, H. NRID 1000010109506 en Kawaoka, Y.
Accessrights	open access
Rights	en Copyright © 1997 American Society for Microbiology
Subject	NDC 649
Description	Abstract en Human influenza viruses are more efficiently isolated by inoculating patient samples into the amniotic rather than the allantoic cavity of embryonated chicken eggs. This type of cultivation selects virus variants with mutations around the hemagglutinin (HA) receptor binding site. To understand the molecular basis of these phenomena, we investigated the abundances of sialic acid (SA) linked to galactose (Gal) by the alpha-2,3 linkage (SA alpha2,3Gal) and SA alpha2,6Gal in egg amniotic and allantoic cells and in Madin-Darby canine kidney (MDCK) cells. Using SA-Gal linkage-specific lectins (Maackia amurensis agglutinin specific for SA alpha2,6Gal and Sambucus nigra agglutinin specific for SA alpha2,3Gal), we found SA alpha2,3Gal in both allantoic and amniotic cells and SA alpha2,6Gal in only the amniotic cells. MDCK cells contained both linkages. To investigate how this difference in abundances of SA alpha2,3Gal and SA alpha2,6Gal in allantoic and amniotic cells affects the appearance of host cell variants in eggs, we determined the receptor specificities and HA amino acid sequences of two different patient viruses which were isolated and passaged in the amnion or in the allantois and which were compared with MDCK cell-grown viruses. We found that the viruses maintained high SA alpha2,6Gal specificities when grown in MDCK cells or following up to two amniotic passages; however, further passages in either the amnion or allantois resulted in the acquisition of, or a complete shift to, SA alpha2,3Gal specificity, depending on the virus strain. This change in receptor specificity was accompanied by the appearance of variants in the population with Leu-to-Gln mutations at position 226 in their HA. These findings suggest that lack of SA alpha2,6Gal linkages in the allantois of chicken eggs is a selective pressure for the appearance of host cell variants with altered receptor specificities and amino acid changes at position 226.
Publisher	en American Society for Microbiology
Date	Issued1997-04
Language	eng
Resource Type	journal article
Version Type	VoR
Identifier	HDL http://hdl.handle.net/2115/28142
Relation	isIdenticalTo DOI https://doi.org/10.1128/jvi.71.4.3357-3362.1997 PMID 9060710
Journal	PISSN 0022-538X en Journal of Virology Volume Number71 Issue Number4 Page Start3357 Page End3362
File	fulltext JV71-4.pdf 188.16 KB (application/pdf) Issued1997-04
Oaidate	2023-07-26