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Title
  • en Enhanced production of p24 Gag protein in HIV-1-infected rat cells fused with uninfected human cells.
Creator
    • en Chen, Jing
    • en Zhao, Xudong
    • en Lai, Yurong
    • en Suzuki, Akira
    • en Tomaru, Utano
    • en Takada, Akio
    • en Ikeda, Hitoshi
    • en Kasahara, Masanori
    • en Yoshiki, Takashi
Accessrights open access
Subject
  • Other en HIV-1
  • Other en Rat model
  • Other en Cell fusion
  • Other en Cyclin T1
  • Other en CDK9
  • Other en CRM1
  • Other en HP68
  • Other en CIITA
  • NDC 490
Description
  • Abstract en Although many human molecules have been suggested to affect replication of human immunodeficiency virus type 1 (HIV-1), the distribution of such cofactors in human cell types is not well understood. Rat W31/D4R4 fibroblasts expressing human CD4 and CXCR4 receptors were infected with HIV-1. The provirus was integrated in the host genome, but only a limited amount of p24 Gag protein was produced in the cells and culture supernatants. Here we found that p24 production was significantly increased by fusing HIV-1-infected W31/D4R4 cells with uninfected human cell lines of T-cell, B-cell, or macrophage lineages. These findings suggest that human cellular factors supporting HIV-1 replication are distributed widely in cells of lymphocyte and macrophage lineages. We also examined whether the amount of p24 produced by rat–human hybrid cells was correlated with expression levels of specific human genes. The results suggested that HP68 and MHC class II transactivator (CIITA) might up- and down-regulate p24 production, respectively. It was also suggested that HIV-1 replication is affected by molecules other than those examined in this study, namely, cyclin T1, cyclin-dependent kinase 9, CRM1, HP68, and CIITA.
Publisher en Elsevier Inc.
Date
    Issued2007-08
Language
  • eng
Resource Type journal article
Version Type AM
Identifier HDL http://hdl.handle.net/2115/28263
Relation
  • URI http://www.sciencedirect.com/science/journal/00144800
  • isVersionOf DOI https://doi.org/10.1016/j.yexmp.2006.11.003
  • PMID 17222823
Journal
    • PISSN 0014-4800
    • EISSN 1096-0945
      • en Experimental and Molecular Pathology
      • Volume Number83 Issue Number1 Page Start125 Page End130
File
    • fulltext EMP83-1.pdf
    • 307.5 KB (application/pdf)
      • Issued2007-08
Oaidate 2023-07-26